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Analysis

1.WO/2022/139447PURIFICATION APPARATUS OF BIOPARTICLES WITH SURFACE CHARGES, AND PURIFICATION METHOD OF BIOPARTICLES BY USING SAME
WO 30.06.2022
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/KR2021/019589 Applicant KOOKMIN UNIVERSITY INDUSTRY ACADEMY COOPERATION FOUNDATION Inventor DO, Young Rag
The present invention relates to a purification apparatus of bioparticles with surface charges and to a purification method of bioparticles by using same and, more particularly, to a bioparticle purification apparatus and purification method which are capable of realizing high purification efficiency and speed while minimizing deformation or damage of bioparticles during a purification process.
2.WO/2022/140482ENERGY FROM BACTERIA AND SEABED EXTRACTION
WO 30.06.2022
Int.Class C02F 1/00
CCHEMISTRY; METALLURGY
02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
1Treatment of water, waste water, or sewage
Appl.No PCT/US2021/064749 Applicant RAYTHEON BBN TECHNOLOGIES, CORP. Inventor ROSENTHAL, Benjamin, Jacob
A method of generating hydrogen gas includes providing a colony of sulfur-reducing bacteria and a colony of sulfur-oxidizing bacteria. The colonies can be submerged in a body of water. The colony of sulfur-reducing bacteria can be used to convert at least a portion of sulfates present in the body of water to hydrogen sulfide. The colony of sulfur-oxidizing bacteria can be used to convert the hydrogen sulfide to sulfuric acid. The sulfuric acid can react with manganese to produce hydrogen gas and manganese sulfate.
3.WO/2022/134062SUBSTRATE, MICROFLUIDIC DEVICE, DRIVING METHOD AND MANUFACTURING METHOD
WO 30.06.2022
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Appl.No PCT/CN2020/139593 Applicant BOE TECHNOLOGY GROUP CO., LTD. Inventor FAN, Bolin
A substrate for a microfluidic device, a microfluidic device, a driving method for a microfluidic device, and a method for manufacturing a substrate for a microfluidic device. A first substrate (102) comprises a first electrode layer (183) located on a first base (180), the first electrode layer (183) comprising a plurality of driving electrodes (170). The plurality of driving electrodes (170) defines at least one flow channel (128, 121, 132, 142, 154, 162) and at least one functional zone (112, 120, 130, 140, 150, 160) in the first substrate (102), the at least one functional zone (112, 120, 130, 140, 150 and 160) comprises a reagent zone (112), and the reagent zone (112) comprises a reagent zone liquid storage portion (204) and a droplet shape changing portion (205). The droplet shape changing portion (205) is adjacent to a reagent zone flow channel (206), and the reagent zone liquid storage portion (204) is located at the side of the droplet shape changing portion (205) away from the reagent zone flow channel (206). The reagent zone liquid storage portion (204), the droplet shape changing portion (205) and the reagent zone flow channel (206) are configured to generate an intermediate droplet covering the droplet shape changing portion (205) from the reagent zone liquid storage portion (204), and are configured to change the shape of the intermediate droplet so as to generate a reagent droplet in the reagent zone flow channel (206).
4.WO/2022/137302DESIGN METHOD AND MANUFACTURING METHOD FOR LIQUID HANDLING FACILITY, AND LIQUID HANDLING FACILITY
WO 30.06.2022
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/JP2020/047757 Applicant JGC JAPAN CORPORATION Inventor KUDO, Gaku
Provided is a method for efficiently designing a liquid handling facility comprising a container for storing a liquid and pipes provided with a plurality of valves. This method for designing a liquid handling facility which stores a liquid in a container 2a and handles the liquid includes: a step for pre-designing valve blocks which are combined so as to integrate a plurality of valves for executing the operation of a fluid and interposed in the plurality of pipes connected to the container 2a, a plurality of connecting pipe parts, and a support structure; a step for selecting at least one valve block having a required function from a valve block group including a plurality of different valve blocks having mutually differing functions; and a step for designing the plurality of pipes in which the selected valve block is interposed and which are connected to the container.
5.WO/2022/137752CELL CULTURING VESSEL AND CELL CULTURING APPARATUS
WO 30.06.2022
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/JP2021/038015 Applicant SHIMADZU CORPORATION Inventor INOUE, Tsunehiro
A cell culturing vessel according to the present invention comprises: an insert member that has a membrane on which cells are seeded and defines a first internal space that functions as an anaerobic chamber; a vessel that has an attachment/detachment part, to which the insert member is attached and from which the insert member is detached, and that defines a second internal space that functions as an aerobic chamber; a seal member that closes an opening of the aerobic chamber and is present between the attachment/detachment part and the insert member in a state in which the insert member is attached to the attachment/detachment part; and a transmission mechanism that transmits force to the seal member. The seal member is configured so as to close the opening by receiving the force from the transmission mechanism.
6.20220205925Flow Cell System for Optical Fluid Analysis and Bioreactor System
US 30.06.2022
Int.Class G01N 21/85
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
84Systems specially adapted for particular applications
85Investigating moving fluids or granular solids
Appl.No 17262363 Applicant Mathias Belz Inventor Mathias Belz

A flow cell system for an optical fluid analysis comprises a disposable flow cell having at least one flow chamber comprising a fluid pathway, and at least one pair of opposed light transmitting windows along the fluid pathway, an external flow cell holder for holding the flow cell, at least one light source, and an external detection device couplable with at least one of the flow cell holder and the flow cell for bringing the external detection device in optical communication with the flow cell, the device having at least one optical detection unit. The external detection device is configured to conduct optical measurements of the fluid that flows in the flow cell through at least one pair of windows from externally under illumination by the at least one light source.

7.20220208297PREDICTING CELL CULTURE PERFORMANCE IN BIOREACTORS
US 30.06.2022
Int.Class G16B 5/00
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
5ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
Appl.No 17599656 Applicant AMGEN INC. Inventor William Leigh Johnson

A computational method of modeling a bioreactor combines mechanistic models of kinetics of metabolic fluxes and flux balance analysis to predict cell culture performance. The mechanistic models include effects of process variables descriptive of extracellular environment, e.g., temperature, acidity, osmolarity, and/or metabolite concentrations. The method constrains flux rates based on the mechanistic models and computes the flux rates in view of suitable metabolic objectives. The method simulates time evolution of process variables of the bioreactor based on user input and computes performance metrics to display to the user, to control a bioreactor, and/or to train an artificial intelligence model of a bioreactor.

8.WO/2022/138351TUMOR ANALYSIS SYSTEM
WO 30.06.2022
Int.Class C12M 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
Appl.No PCT/JP2021/046157 Applicant HITACHI, LTD. Inventor ABE Sachiho
The present invention provides a system for analyzing the composition of cells in a tumor specimen. This system is characterized by including at least a calculation unit and is characterized in that at least one storage apparatus included in the system or connected to the system includes: the cell composition of a cancer clone obtained from cells included in the tumor specimen on the basis of gene mutation information; and the cell composition of normal cells obtained from cells included in the tumor specimen on the basis of a gene expression level, the system also being characterized in that the calculation unit executes: a step for adjusting the abundance ratios of each cell species, such that the total of the abundance ratios of each cell species is X (X is a constant of 0 to 1 inclusive and is a parameter designated by a user of the system) with respect to the cell composition of the cancer clone and the cell composition of the normal cells read out from the storage apparatus while taking it into consideration that normal cells are included in the unclassified cells out of the cell composition of the cancer clone; and a step for outputting the cell composition of the tumor specimen after the adjustment.
9.WO/2022/137061METHODS AND SYSTEMS FOR IMPROVED CELL TRANSFECTION
WO 30.06.2022
Int.Class C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
Appl.No PCT/IB2021/061952 Applicant PFIZER INC. Inventor DETERMAN, Larry Dean
The present disclosure provides improved methods and systems for transfecting host cells with nucleic acids, such as plasmid DNA, for purposes of efficiently producing biological products, such as AAV vectors, at large scale.
10.20220205013APPARATUS AND METHOD FOR DISTINGUISHING ANTIBIOTICS SUSCEPTIBILITY
US 30.06.2022
Int.Class C12Q 1/18
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
18Testing for antimicrobial activity of a material
Appl.No 17386687 Applicant ACCUNOSE CO.,LTD. Inventor Won Il LEE

A method of testing an antibiotic susceptibility includes dispensing and cultivating sample solution into culture wells including one or more comparative wells and a plurality of antibiotic wells receiving two or more kinds of antibiotics, respectively, receiving the sample solution into a plurality of preprocessing wells each including magnetic particles and fluorescent particles that bond to one or more kinds of bacteria such that the bacteria and the magnetic particles and fluorescent particles bond to each other, receiving the sample solution into a plurality of image wells having magnetic members thereunder such that the magnetic particles bonding to the bacteria are arranged on the bottoms of the image wells, removing the sample solution from the image wells that have undergone the planarizing step, taking fluorescent images of the image wells washed in the washing step, and determining an antibiotic tolerance/susceptibility of the sample solution by analyzing the fluorescent images.