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Analysis

1.WO/2022/169899SWAB COLLECTION MEDIA FOR CAPTURE OF AIRBORNE PARTICLE SAMPLES
WO 11.08.2022
Int.Class G01N 1/22
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
22in the gaseous state
Appl.No PCT/US2022/014976 Applicant AEROSOL DEVICES INC Inventor HESKETT, Dominick Dalton Reyes
The present invention features a device and method for directly collecting airborne particles onto a swab collection substrate. Prior to collection, water vapor may be condensed onto the particles to increase their average diameter. The particles are expelled from one or more acceleration nozzles for gentle impaction onto the swab collection substrate and may be further analyzed through chemical or biological assays.
2.20220251671SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF AN ANALYTE, SUCH AS SARS-COV-2, IN A SAMPLE
US 11.08.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 17666338 Applicant ADL Diagnostics Inventor Robert G. ATKINSON

Methods for detecting an analyte in a sample are disclosed. The method can include depositing the sample in an instrument, such as a Loop-Mediated Isothermal Amplification (LAMP) instrument that is configured to selectively amplify an analyte, such as a characteristic portion of a genome of a pathogen. A moving average of the quantity of the analyte at an instance of time can be compared to a sum of (1) the moving average for a previous instance of time and (2) a multiple of the moving standard deviation at the previous instance of time. If the quantity of the analyte at the instance of time is greater than the sum of (1) the moving average for a previous instance of time and (2) a multiple of the moving standard deviation at the previous instance of time, it can be an indication that the sample is positive for the analyte.

3.WO/2022/170013MULTIPLEXED, CRISPR-BASED DIAGNOSTICS OF SARS-COV-2 IN AUTONOMOUS MICROFLUIDIC DEVICE
WO 11.08.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/US2022/015175 Applicant UNIVERSITY OF CONNECTICUT Inventor LIU, Changchun
Described herein is an autonomous, rapid, sensitive, point-of-care target nucleic acid detection system and method based on CRISPR/Cas system. The system and method allow naked eye visualization of multiple target nucleic acid molecules simultaneously in a biological sample in less than an hour. Use of the system and method for multiplex gene diagnosis of SARS-CoV-2 is exemplified.
4.WO/2022/167570COMPOSITIONS AND METHODS FOR DETECTION OF HUMAN PARAINFLUENZA VIRUSES 1-4 (HPIV 1-4)
WO 11.08.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/EP2022/052689 Applicant F. HOFFMANN-LA ROCHE AG Inventor MANOHAR, Chitra
Methods for the rapid detection of the presence or absence of Human Parainfluenza Viruses (HPIV), including HPIV 1-4 in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting HPIV 1-4, and kits are provided that are designed for the detection of target regions of HPIV 1-4. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of HPIV 1-4.
5.WO/2022/167795VIRAL LOAD TESTER AND APPLICATIONS THEREOF
WO 11.08.2022
Int.Class G01N 21/64
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
Appl.No PCT/GB2022/050277 Applicant SILVER, Joshua D. Inventor SILVER, Joshua D.
The present invention provides a system (100) and method for measuring an amount of virus (106) in a sample (104) to be tested. The system (100) comprises a light emitting diode (102) operable to emit UV light towards a sample to be tested, and a detector (108) operable to detect light from fluorescence events induced in a sample by UV light emitted from the light emitting diode (102). An amount of virus (106) in the sample is then estimated based on at least the light from fluorescence events that is detected by the detector (108).
6.20220251519CONCENTRATION MEMBRANE, CONCENTRATION DEVICE, CONCENTRATION SYSTEM, AND CONCENTRATION METHOD FOR BIOLOGICAL PARTICLES, AND METHOD FOR DETECTING BIOLOGICAL PARTICLES
US 11.08.2022
Int.Class C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
Appl.No 17438077 Applicant TEIJIN LIMITED Inventor Mami NAMBU

A concentration membrane for use in concentrating biological particles, including: a hydrophilic composite porous membrane including: a porous substrate; and a hydrophilic resin with which at least one main surface and inner surfaces of pores of the porous substrate are coated, the hydrophilic composite porous membrane having a ratio t/x of a membrane thickness t (m) to an average pore diameter x (m), as measured with a perm porometer, of from 50 to 630. A concentration device 10 for biological particles 50 including: a housing 20 having an inlet 21 and an outlet 22, in which, due to a differential pressure between the inlet 21 and the outlet 22, a liquid to be treated 40 containing biological particles 50 and water is injected from the inlet 21 and discharged from the outlet 22; a concentration membrane 30 provided to separate the inlet 21 and the outlet 22 from each other in the housing 20, the concentration membrane 30 being a hydrophilic porous membrane onto which the biological particles 50 are not adsorbed, the concentration membrane 30 allowing an effluent 42, which is a liquid having a concentration that is a concentration of the biological particles 50 subtracted from a concentration of the liquid to be treated 40, to permeate from a surface on a side of the inlet 21 to a surface on a side of the outlet 22; and a concentration space portion 24 which is a space on an upstream side of the concentration membrane 30 in the housing 20 and stores a concentrated liquid 41 which is a liquid having a concentration that is a concentration of the biological particles 50 added to a concentration of the liquid to be treated 40 by the concentration membrane 30.

7.WO/2022/170202SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF AN ANALYTE, SUCH AS SARS-COV-2, IN A SAMPLE
WO 11.08.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/US2022/015512 Applicant ADL DIAGNOSTICS Inventor ATKINSON, Robert G.
Methods for detecting an analyte in a sample are disclosed. The method can include depositing the sample in an instrument, such as a Loop-Mediated Isothermal Amplification (LAMP) instrument that is configured to selectively amplify an analyte, such as a characteristic portion of a genome of a pathogen. A moving average of the quantity of the analyte at an instance of time can be compared to a sum of (1) the moving average for a previous instance of time and (2) a multiple of the moving standard deviation at the previous instance of time. If the quantity of the analyte at the instance of time is greater than the sum of (1) the moving average for a previous instance of time and (2) a multiple of the moving standard deviation at the previous instance of time, it can be an indication that the sample is positive for the analyte.
8.WO/2022/168418TEST INSTRUMENT, TEST KIT AND TEST METHOD
WO 11.08.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/JP2021/044116 Applicant ICST CORPORATION Inventor SEKI Masaaki
The purpose of the present invention is to provide a test instrument and a test method using immunochromatography, by which the efficacy of vaccination against a specific virus such as new coronavirus can be rapidly and easily determined. A test instrument 10, which uses immunochromatography, comprises a fist test strip 11, a second test strip 12 which is different from the first test strip 11, and a case 13 in which the first and second test strips 11 and 12 are integrally housed. The case 13 is provided with a specimen drop window 103, a first detection window 131 through which a portion of the first test strip 11 is visible from the outside, and a second detection window 132 through which a portion of the second test strip 12 is visible from the outside. The first test strip 11 enables the detection of the presence of a first antibody in a specimen. The second test strip 12 enables the detection of the presence of a second antibody, which is different from the first antibody, in the specimen. Due to this structure, the result of the first test strip 11 visually confirmed through the first detection window 131 and the result of the second test strip 12 visually confirmed through the second detection window 132 can be determined simultaneously in parallel.
9.WO/2022/167360ATTENUATED AFRICAN SWINE FEVER VIRUS AND ITS USE AS A VACCINE
WO 11.08.2022
Int.Class C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
Appl.No PCT/EP2022/052183 Applicant AGENCE NATIONALE DE SECURITE SANITAIRE DE L'ALIMENTATION DE L'ENVIRONNEMENT ET DU TRAVAIL Inventor BLOT LE POTIER, Marie-Frédérique
The present invention relates to an attenuated African Swine Fever (ASF) virus, wherein : • genes MGF 360-12L, 360-13L, 360-14L, 505-2R, 505-3R are deleted or are interrupted or mutated such that the genes are not transcribed and/or translated, • ORF of ASFV_G_ACD_00520 is deleted or is interrupted or mutated such that it is not transcribed and/or translated, and • genes MGF 505-1 R et 505-4R are truncated, compared to the genome of the corresponding unattenuated virus. The present invention also refers to a vaccine comprising the attenuated ASF virus, and its use in preventing African Swine Fever in a subject. The present invention also relates to an in-vitro method for obtaining the attenuated ASF virus, which comprises at least one step of thermal- attenuation of a virulent ASFV virus strain selected among Georgia 2007/1, Pig/HLJ/2018, a strain of ASF virus of genotype II or a genetically close ASF virus strain, and amplification by inoculation of Specific-Pathogen- Free pigs and selecting said attenuated ASF virus. The present invention refers to an in vitro method for the differential detection of the attenuated ASF virus and of the corresponding non- attenuated ASF virus as well.
10.WO/2022/170116METHODS AND SYSTEMS FOR THE RAPID DETECTION OF LISTERIA USING INFECTIOUS AGENTS
WO 11.08.2022
Int.Class C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
Appl.No PCT/US2022/015345 Applicant LABORATORY CORPORATION OF AMERICA HOLDINGS Inventor ERICKSON, Stephen
Disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria- specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.