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IC:C12Q1/68 AND EN_ALLTXT:(coronavirus OR coronaviruses OR coronaviridae OR coronavirinae OR orthocoronavirus OR orthocoronaviruses OR orthocoronaviridae OR orthocoronavirinae OR betacoronavirus OR betacoronaviruses OR betacoronaviridae OR betacoronavirinae OR sarbecovirus OR sarbecoviruses OR sarbecoviridae OR sarbecovirinae OR "severe acute respiratory syndrome" OR sars OR "2019 ncov" OR covid)

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Analysis

1.20220243290MOLECULAR DETECTION OF NOVEL CORONAVIRUSES
US 04.08.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 17545818 Applicant The University of Hong Kong Inventor Leo LM Poon

Systems for sensitive and accurate detection of sarbecoviruses in samples from human sputum have been established. The systems can rapidly and effectively diagnose patients having suspected coronavirus infections. The systems employ Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) to identify and quantify nucleic acids specific to sarbecoviruses within samples from human patients. The systems identify viral RNAs specific for the N and ORF1 genes using a two-step real-time PCR and the SYBR Green detection method. Compositions and methods for reproducibly detecting and quantifying concentrations of sarbecoviruses with a dynamic range of at least seven orders of magnitude (2×10−4-2000 TCID50/reaction) are described. The compositions and methods enable rapid early diagnosis of patients, as well as development of databases of patient viral titres and infectious dosages.

2.WO/2021/226278AN ULTRASENSITIVE RAPID AND PORTABLE CASE13D-BASED DIAGNOSTIC ASSAY
WO 11.11.2021
Int.Class A61K 38/46
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
43Enzymes; Proenzymes; Derivatives thereof
46Hydrolases (3)
Appl.No PCT/US2021/030953 Applicant THE REGENTS OF THE UNIVERSITY OF CALIFORNIA Inventor AKBARI, Omar
Provided herein is a viral RNA detection system, utilizing the RNA-targeting properties of the optimized Casl3d enzyme, CasRx, to detect SARS-CoV-2 RNA, e.g., synthetic SARS- CoV-2 RNA. The system detects novel target sequences conserved within the actively evolving genome, to provide a panel of diagnostic target sites least likely to result in false negatives due to genomic variation. Successful detection of viral RNA through both a fluorescence-based readout assay as well as a rapid paper dipstick lateral flow assay requiring no specialized laboratory equipment was shown. Low viral titers can be detected within minutes following only minutes of sample processing.
3.WO/2021/229582DEVICE, SYSTEMS, KITS AND METHODS FOR RAPID AND SIMPLE DETECTION OF PATHOGENS
WO 18.11.2021
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Appl.No PCT/IL2021/050557 Applicant TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD. Inventor GEVA-ZATORSKY, Naama
The present disclosure provides devices, systems, kits and methods for rapid, simple self-sampling and self-performing detection of nucleic acid sequence of interest in a sample, specifically, nucleic acid sequences of pathogenic agents.
4.WO/2022/085007DEVICE, SYSTEMS AND METHODS FOR DETECTION OF NUCLEIC ACID MOLECULES OF INTEREST
WO 28.04.2022
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Appl.No PCT/IL2021/051253 Applicant TECHNION RESEARCH & DEVELOPMENT FOUNDATION LTD. Inventor ROFMAN, Baruch
A sample testing module, for use with a device for enabling detection and monitoring of at least one target element in a sample, includes a reaction pad and a support. The reaction pad includes a preparation zone that is contiguous with a plurality of reaction zones via respective branch elements. The preparation zone is configured for: accommodating sample preparation agents and at least a sample to be tested, and for enabling mixing thereof to provide a prepared sample; accommodating a second quantity of a transporting liquid and for enabling contact of the prepared sample with the transporting liquid to provide the flowable prepared sample. Each reaction zone is configured for accommodating therein a respective reaction mixture adapted for the reaction, to produce at least one respective product. The support includes a first heating system (for enabling heating of the preparation zone to a first temperature) and a second heating system (for enabling heating of the amplification zones to a second temperature).
5.10815539Assays for the detection of SARS-CoV-2
US 27.10.2020
Int.Class C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
Appl.No 16837364 Applicant DiaSorin S.p.A. Inventor Chiara Brambati

The present invention is directed to methods for assaying for the presence of SARS-CoV-2 in a sample, including a clinical sample, and to oligonucleotides, reagents and kits useful in such assays. In particular, the present invention is directed to such assays that are rapid, accurate and specific for the detection of SARS-CoV-2.

6.WO/2022/147868SEWAGE SURVEILLANCE FOR SARS-COV-2
WO 14.07.2022
Int.Class C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
Appl.No PCT/CN2021/074675 Applicant THE UNIVERSITY OF HONG KONG Inventor ZHANG, Tong
Systems for sensitive and accurate detection ofinfectious agents in samples from sewage and wastewater have been established. The systems can rapidly and effectively detect and record the presence ofSARS-CoV-2 and other viruses within wastewater and sewage from specific locations. The systems employ Quantitative Polymerase Chain Reaction (RT-qPCR) to identify and quantify nucleic acids indicative ofSARS-CoV-2 and other viruses within samples obtained from sewagesheds. The systems reproducibly and accurately identify and quantitate viral RNAs for enable rapid and early monitoring ofmunicipal water systems in areas housing or related to patients infected with SARS-CoV-2 and/or other viruses, and provide databases to monitor the presence ofinfectious agents within sewagesheds on a community-wide level.
7.20210285061COMPOSITIONS AND METHODS FOR DETECTING SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (SARS-COV-2), INFLUENZA A AND INFLUENZA B
US 16.09.2021
Int.Class C12Q 1/6888
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
Appl.No 17249645 Applicant Roche Molecular Systems, Inc. Inventor Chitra Manohar

Methods for the rapid detection of the presence or absence of SARS-CoV-2, influenza A and influenza B in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting SARS-CoV-2, influenza A, and influenza B and kits are provided that are designed for the detection of SARS-CoV-2, influenza A and influenza B.

8.20210310060METHODS AND COMPOSITIONS FOR THE DIAGNOSIS AND TREATMENT OF COVID 19
US 07.10.2021
Int.Class C12Q 1/6853
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6853using modified primers or templates
Appl.No 17209985 Applicant PRESIDENT AND FELLOWS OF HARVARD COLLEGE Inventor Connie CEPKO

Described herein are methods and compositions related to the diagnosis and treatment of COVID 19 and the detection of SARS-Cov-2.

9.WO/2021/195277MULTIPLEXED METHODS FOR DETECTING TARGET RNAS
WO 30.09.2021
Int.Class C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
Appl.No PCT/US2021/023978 Applicant PRESIDENT AND FELLOWS OF HARVARD COLLEGE Inventor KIRSCHNER, Marc W.
The technology described herein is directed to methods, kits, compositions, and systems for detecting a target RNA, such as a small amount of viral RNA. In one aspect, described herein are methods of detecting the target RNA, using primers comprising at least one barcode region. In other aspects, described herein are kits, compositions, and systems suitable to practice the methods described herein to detect the target RNA.
10.WO/2022/018685COMPOSITIONS AND METHODS FOR ONE-STEP NUCLEIC ACID AMPLIFICATION AND DIAGNOSTIC METHODS BASED THEREON
WO 27.01.2022
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/IB2021/056646 Applicant KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY Inventor HAMDAN, Samir M.
Compositions and methods for a nucleic acid amplification and diagnostic methods based therein, are disclosed. The compositions include purified Thermus aquaticus DNA polymerase (Taq Pol) and Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT), in proportions and under reaction conditions that allow one-step amplification of a nucleic acid of interest. In particular, the compositions are useful in one-step RT-qPCR and RT-LAMP methods. The method includes sampling the specimen from a subject, extracting and purifying RNA from the sample. The compositions and methods may be used to produce, analyze, quantitate, detect and otherwise manipulate nucleic acid molecules. For example, disclosed are methods of detecting the presence of a viral nucleic acid in a sample from a subject. Methods of diagnosing a subject as being infected with a viral pathogen are also provided. A preferred virus or pathogen is SARS-CoV-2.