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1.WO/2022/166103TRANSAMINASE MUTANT AND APPLICATION THEREOF
WO 11.08.2022
Int.Class C12N 9/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
Appl.No PCT/CN2021/104841 Applicant ASYMCHEM LABORATORIES (TIANJIN) CO., LTD. Inventor HONG, Hao
Provided are a transaminase mutant and an application thereof. The transaminase mutant is obtained by mutating one or more amino acids in SEQ ID NO: 2 or is a mutant obtained by taking the sequence SEQ ID NO: 1 of wild-type CVTA transaminase as a reference and mutating conserved amino acids therein. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis can be improved.
2.WO/2022/166838CONSTRUCTION AND APPLICATIONS OF ENATIOSELECTIVE FLIPPED ω-TRANSAMINASE MUTANT
WO 11.08.2022
Int.Class C12N 9/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
Appl.No PCT/CN2022/074737 Applicant ZHEJIANG APELOA KANGYU PHARMACEUTICAL CO., LTD. Inventor LIN, Shuangjun
Provided is a transaminase mutant, having a sequence on which an amino acid mutation takes place on the basis of the sequence as represented by SEQ ID NO: 13. Provided is an enatioselective flipped ω-transaminase ATA-117 mutant. The amino acid sequence of the mutant is as represented by SEQ ID NO: 1; the nucleic acid sequence coding the mutant is as represented by SEQ ID NO: 2. Also provided is a plasmid carrying the nucleic acid sequence, genetically engineered bacteria expressing the ω-transaminase ATA-117 mutant, and a method for producing (1R,2R)-1,3-dihydroxy-2-amino-1-p-methylsulfonylphenylpropane with the ω-transaminase ATA-117 mutant.
3.WO/2022/168413METHOD FOR PRODUCING DEPRESTATIN-CONTAINING COMPOSITION
WO 11.08.2022
Int.Class A23L 33/18
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D91; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
33Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
10using additives
17Amino acids, peptides or proteins
18Peptides; Protein hydrolysates
Appl.No PCT/JP2021/043685 Applicant UHA MIKAKUTO CO., LTD. Inventor KASHIMA Yasuhiro
The present invention pertains to a method for producing a deprestatin-containing composition, said method comprising an enzymatic treatment step for hydrolyzing a starting solution containing soy proteins with a protease while elevating temperature in a temperature range of 5-70°C. The temperature elevation may be controlled in two or more steps. The starting solution may be a liquid mixture with water containing crushed soybeans, a press juice or a purified product thereof. By blending the deprestatin-containing composition thus obtained into a food, a nutritionally enriched food composition can be prepared.
4.WO/2022/169793ENZYMATIC ENRICHMENT OF FOOD INGREDIENTS FOR SUGAR REDUCTION
WO 11.08.2022
Int.Class C12N 9/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
90Isomerases (5.)
Appl.No PCT/US2022/014819 Applicant BONUMOSE, INC. Inventor WICHELECKI, Daniel, Joseph
The invention relates to preparation of food ingredients enriched with a low-glycemic sugar replacement through enzymatic conversion. Food ingredients may be enriched with, for example, D-tagatose, D-allulose, D-allose, D-mannose, D-talose, and/or inositol by enzymatically converting saccharides found in flour, meal, ground tuber, ground pulse, ground bark, starch, malted grain or malt extract, maltodextrin, cellulose, cellodextrin, any of their derivatives (e.g., amylose, amylopectin, dextrin, cellobiose, etc.), and/or sucrose into D-tagatose, D-allulose, D-allose, D-mannose, D-talose and/or inositol. The enriched material can be used as a food ingredient instead of the low-glycemic sugar being purified for use as a food ingredient.
5.WO/2022/169283COMPOSITION COMPRISING LACTOBACILLUS PLANTARUM-DERIVED POLYSACCHARIDE OR EXTRACT
WO 11.08.2022
Int.Class C12P 19/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Appl.No PCT/KR2022/001731 Applicant NEOREGEN BIOTECH Inventor SEO, Jeong Min
The present invention relates to a composition comprising Lactobacillus plantarum-derived polysaccharide or extract and, more specifically, by comprising Lactobacillus plantarum-derived polysaccharide or a Lactobacillus plantarum extract, may exhibit inflammatory inhibition, adipogenesis inhibition, intracellular glucose absorption inhibition, insulin resistance reduction and hepatic steatosis reduction effects, and may exhibit effects of treating or preventing a metabolic disease.
6.20220251502METHODS FOR REDUCING THE OXIDATION LEVEL OF CYSTEINE RESIDUES IN A SECRETED RECOMBINANTLY-EXPRESSED PROTEIN DURING CELL CULTURE
US 11.08.2022
Int.Class C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
Appl.No 17557921 Applicant NOVARTIS AG Inventor Nuno BUXO CARINHAS

The present disclosure relates to methods for reducing the oxidation level of cysteine residues in recombinant polypeptides such as anti-IL-17 antibodies during cell culture (e.g., a preparation of secukinumab antibodies) that have been recombinantly produced by mammalian cells. Also provided are purified preparations of recombinant polypeptides such as anti-IL-17 antibodies or antigen binding fragments thereof produced by such methods, e.g, purified preparations of secukinumab. Also provided are purified preparations of recombinant polypeptides produced by such methods wherein the level of active recombinant polypeptide in the preparation is high.

7.20220251535AMINOACYL-TRNA SYNTHETASES AND USES HEREOF
US 11.08.2022
Int.Class C12N 9/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
Appl.No 17431452 Applicant Novo Nordisk A/S Inventor Hongtao Guan

The invention relates to aminoacyl-tRNA synthetases that aminoacylate tRNA with 2-Aminoisobutyric acid (Aib), thus enabling the incorporation of the Aib into a growing polypeptide chain during translation, e.g. in eubacterial host cells such as E. coli. For example, but not limited to, the invention relates to new aminoacyl-tRNA synthetases and the uses hereof, as well as a method for producing polypeptides that contain one or more Aib.

8.20220251582MODIFIED YEAST AND METHOD FOR INCREASING LYSINE CONTENT IN FERMENTATION CO-PRODUCTS
US 11.08.2022
Int.Class C12N 15/81
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
Appl.No 17621982 Applicant DANISCO US INC Inventor Jaclyn Diana DEMARTINI

Described are strains and methods relating to genetically-engineered yeast cells that overproduce lysine in a tunable manner by altering feedback inhibition of the lysine synthetic pathway by way of the LYS20 and LYS21 homocitrate synthase polypeptides. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of lysine, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients.

9.WO/2022/165609RECOMBINANT PLANT-DERIVED ANTIBODIES AND FC VARIANTS AND RELATED METHODS
WO 11.08.2022
Int.Class C07K 16/12
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
12against material from bacteria
Appl.No PCT/CA2022/050177 Applicant HER MAJESTY THE QUEEN IN RIGHT OF CANADA, AS REPRESENTED BY THE MINISTER OF AGRICULTURE AND AGRI-FOOD Inventor MENASSA, Rima
The present application describes polypeptides comprising a variant Fc chain that exhibits enhanced accumulation in an organism and methods of producing the same. A method of enhancing accumulation of a protein is also described. The present application also describes polypeptides which specifically bind to intimin on an Escherichia coli cell, including antibodies and antigen-binding fragments thereof, and methods of producing the same. Also described is a method of preventing or reducing colonization of E. coli in the gastrointestinal tract of a mammal. A method of detecting the presence of E. coli in a sample is also described.
10.WO/2022/165954NUTRITIONAL ESSENCE SYRUP OF DATONG HEMEROCALLIS CITRINA AND PREPARATION PROCESS THEREFOR
WO 11.08.2022
Int.Class A23L 33/105
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D91; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
33Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
10using additives
105Plant extracts, their artificial duplicates or their derivatives
Appl.No PCT/CN2021/083512 Applicant SHANXI PROVINCIAL ACADEMY OF AGRICULTURAL SCIENCES, INSTITUTE OF EDIBLE FUNGI Inventor GUO, Shang
A method for preparing a liquid product of a Datong Hemerocallis citrina extract, comprising the following steps: A) performing enzymolysis on raw materials consisting of Cordyceps militaris, Hemerocallis citrina, and Panicum miliaceum by using papain to obtain an enzymolysis product, namely the Datong Hemerocallis citrina extract; and B) collecting liquid components of the Datong Hemerocallis citrina extract, and preparing the liquid product of the Datong Hemerocallis citrina extract by using the liquid components of the Datong Hemerocallis citrina extract. The liquid product prepared by the method contains 18 common amino acids, wherein the content of 8 essential amino acids for human body is 120.46-218.71 mg/100 mL, and the content of cordycepin is 13.64-20.46 mg/100 g. The liquid product is a functional beverage that has comprehensive and balanced nutrition, is rich in cordycepin, has zero fat and low calorie, and contains 8 essential amino acids for human body.