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Analysis

1.WO/2022/200333METHOD TO SELECTIVELY PERMEABILIZE AND/OR FRAGMENTIZE CELLS
WO 29.09.2022
Int.Class A61B 18/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
18Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
Appl.No PCT/EP2022/057464 Applicant UNIVERSITEIT GENT Inventor BRAECKMANS, Kevin
The invention relates to a method to selectively permeabilize and/or fragmentize cells. A structure comprising a material and particles able to absorb electromagnetic radiation and cells are brought at close distance from each other. Part of the particles embedded in the structure are exposed to the free surface of the structure. The structure and in particular the particles in the structure are 5 irradiated with electromagnetic radiation to selectively permeabilize and/or fragmentize the cells. Furthermore the invention relates to a structure for use in a photothermal process to selectively permeabilize and/or fragmentize cells.
2.WO/2022/202043CELL CULTURE CONTAINER AND CELL CULTURE METHOD
WO 29.09.2022
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/JP2022/007072 Applicant TOYODA GOSEI CO., LTD. Inventor TAKI Seitaro
A cell culture container provided with a first container, a second container, and a culture membrane retained between the first container and the second container, in which each of the first container and the second container is provided with a first surface having a retention unit on which the culture membrane is retained, a second surface which faces the first surface, a first cell and a second cell which are located adjacent to each other in a second direction that is orthogonal to a first direction that extends from the first surface toward the second surface, a partitioning wall which partitions between the first cell and the second cell and extends from the first surface toward the second surface, a communication unit which is formed between the partitioning wall and the second surface and allows the first cell and the second cell to be communicated with each other, and a third surface which faces the partitioning wall in the second direction and zones the second cell in conjunction with the second surface, and in which an insertion port is formed.
3.WO/2022/202733CELL CULTURING SYSTEM
WO 29.09.2022
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/JP2022/012949 Applicant TERUMO KABUSHIKI KAISHA Inventor IGARASHI, Masatsugu
A cell culturing system (10) includes a processing unit (24) that performs culturing of cells, a reactor installation device (68) in which the processing unit (24) is capable of being installed, a connection circuit (26) that is connected to the processing unit (24), and a circuit control device (66) which the connection circuit (26) is capable of being attached to and detached from, and which is capable of carrying out supply of the cells and a culture medium from the connection circuit (26) to the processing unit (24), and carrying out collection of cultured cells from the processing unit (24) into the connection circuit (26). The processing unit (24) includes a plurality of bioreactors (30). The reactor installation device (68) is disposed separately with respect to the circuit control device (66).
4.WO/2022/203513APPARATUS AND PROCESS FOR CULTURING TISSUE
WO 29.09.2022
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/NL2022/050165 Applicant MEATABLE B.V. Inventor DEZFULI, Sina
An apparatus for culturing muscle tissue comprises a chamber extending from a first end to a second end; a first anchor located within the chamber at the first end; a second anchor located within the chamber at the second end; an inlet; and an outlet. The chamber can have an expanding or cylindrical cross-section from each of the first end and the second end to a point between the first end and the second end. The first and second anchors are three-dimensional.
5.20220306978CELL CAPTURE AND EXPANSION
US 29.09.2022
Int.Class C12M 1/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
12with sterilisation, filtration, or dialysis means
Appl.No 17702658 Applicant Terumo BCT, Inc. Inventor Mark E. Jones

Implementations are described that relate to methods and systems for growing cells in a hollow fiber bioreactor. In implementations, the cells may be exposed to a number of growth factors including a combination of recombinant growth factors. In other implementations, the cells may be grown in co-culture with other cells, e.g., hMSC's. In implementations, the cells may include CD34+ cells. A coated membrane includes a membrane having a first coating configured to promote cellular adhesion to the membrane and a second coating that includes a soluble protein moiety.

6.20220305399PROCESS AND SYSTEM FOR PRODUCT RECOVERY AND CELL RECYCLE
US 29.09.2022
Int.Class B01D 3/10
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
3Distillation or related exchange processes in which liquids are contacted with gaseous media, e.g. stripping
10Vacuum distillation
Appl.No 17806219 Applicant LanzaTech, Inc. Inventor Paul Alvin Sechrist

The invention is directed to a method for recovering at least one product from a fermentation broth. The invention relates to the use of a vacuum distillation vessel to recover products, such as ethanol, from a fermentation broth, where the fermentation broth comprises viable microbial biomass, and where the recovery of the product is completed in such a manner to ensure the viability of the microbial biomass. The invention provides for product recovery at an effective rate so as to prevent the accumulation of product in the fermentation broth. To ensure the viability of the microbial biomass, the invention is designed to reduce the amount of stress on the microbial biomass. By ensuring the viability of the microbial biomass, the microbial biomass may be recycled and reused in the fermentation process, which may result in an increased efficiency of the fermentation process.

7.WO/2022/202044CELL CULTURE VESSEL AND CELL CULTURE METHOD
WO 29.09.2022
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/JP2022/007077 Applicant TOYODA GOSEI CO., LTD. Inventor TAKI Seitaro
Provided is a cell culture vessel including: an inner cylinder having an inner-cylinder one-end section, an inner-cylinder other-end section facing the inner-cylinder one-end section, a cylindrical inner-cylinder side wall connecting the inner-cylinder one-end section and the inner-cylinder other-end section, and an inner-cylinder recessed section formed as a notch in the inner-cylinder other-end section; a liquid stop pin that is detachably attached to the inner-cylinder recessed section to close the inner-cylinder recessed section; a culture membrane part that is disposed on the inner-cylinder one-end section and that closes an inner-cylinder opening formed in the inner-cylinder one-end section; and an outer cylinder that is detachably attached to the inner cylinder from the inner-cylinder one-end section side and that has an outer-cylinder one-end section, an outer-cylinder other-end section facing the outer-cylinder one-end section, and a cylindrical outer-cylinder side wall connecting the outer-cylinder one-end section and the outer-cylinder other-end section. In a state in which the outer cylinder is attached to the inner cylinder, the culture membrane part closes an outer-cylinder opening in the outer-cylinder other-end section.
8.WO/2022/202734CELL CULTURE SYSTEM
WO 29.09.2022
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/JP2022/012950 Applicant TERUMO KABUSHIKI KAISHA Inventor IGARASHI, Masatsugu
This cell culture system (10) is provided with: a treatment unit (24) which cultures cells; a reactor installation device (68) where the treatment unit (24) can be installed; a connection circuit (26) which is connected to the treatment unit (24); a circuit control device (66) to which the connection circuit (26) can be detachably attached; and a tank device (64) which has a culture medium housing unit (74) for housing a culture medium. The treatment unit (24) has multiple bioreactors (30). The culture medium in the culture medium housing unit (74) is supplied to the treatment unit (24) via the connection circuit (26). The culture medium housing unit (74) can house the amount of culture medium necessary to culture cells in the treatment unit (24).
9.WO/2022/202854MIRNAS, COMPOSITIONS, AND METHODS OF USING THEREOF
WO 29.09.2022
Int.Class C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
Appl.No PCT/JP2022/013311 Applicant CRAIF INC. Inventor YAMAGUCHI Hiroki
A method for identifying a patient as having a marker correlated with systemic lupus erythematosus (SLE) comprises obtaining a body fluid sample from a patient suspected of having SLE, analyzing miRNA expression in the obtained body fluid sample, and identifying the patient as having the marker correlated with SLE if an increase in expression of at least one miRNA selected from SEQ ID NOs: 1-160 and 243-402 and/or a decrease in expression of at least one miRNA selected from SEQ ID NOs: 161-242 and 403-484 compared to a body fluid sample obtained from a healthy individual is detected in the patient sample, or as not having the marker correlated with SLE if an increase in expression of at least one miRNA selected from SEQ ID NOs: 1-160 and 243-402 and/or a decrease in expression of at least one miRNA selected from SEQ ID NOs: 161-242 and 403-484 compared to a body fluid sample obtained from a healthy individual fails to be detected.
10.102021107590Vorrichtung zum effizienten Mediumwechsel in Mikrotiterplatten
DE 29.09.2022
Int.Class C12M 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
16containing, or adapted to contain, solid media
18Multiple fields or compartments
20Horizontal planar fields
Appl.No 102021107590 Applicant Heinrich-Heine-Universität Düsseldorf Inventor Esser Knud

Die vorliegende Erfindung betrifft eine Vorrichtung zur simultanen Befüllung von Mikrotiterplatten-Wells mittels Zentrifugalkräften. Die Vorrichtung umfasst einen flächigen Aufsatz für eine Mikrotiterplatte aufweisend ein oder mehrere Flüssigkeitsspeicher zur Befüllung jeweils eines Wells der Mikrotiterplatte pro Flüssigkeitsspeicher, wobei jeder Flüssigkeitsspeicher eine obere Befüllöffnung zur Aufnahme einer Befüllflüssigkeit, eine oder mehrere untere Befülldüsen zur Abgabe der Befüllflüssigkeit in den Well der Mikrotiterplatte und eine die Befüllöffnung und die Befülldüse miteinander verbindende Speicherwand aufweist, wobei die Befülldüse azentrisch zur Symmetrieachse des Mikrotiterplatten-Wells und azentrisch zur Symmetrieachse der Befüllöffnung der Befüllvorrichtung in einem Teilbereich der Speicherwand angeordnet ist. embedded image