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1.WO/2022/007881MODIFIED GLUTAMATE DEHYDROGENASE AND USE THEREOF
WO 13.01.2022
Int.Class C12N 9/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
06acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
Appl.No PCT/CN2021/105179 Applicant SICHUAN LIER BIOTECHNOLOGY CO., LTD. Inventor XIE, Xinkai
Provided is modified glutamate dehydrogenase (GluDH). In particular, the modified GluDH provided has an improved activity of catalyzing PPO to react with an amino donor to generate L-glufosinate-ammonium and/or has an improved kinetic property. Further provided are a polynucleotide for encoding the modified GluDH, a vector and host cell for expressing the modified GluDH of the present invention, and a method for producing L-glufosinate-ammonium by means of using the modified GluDH and the host cell.
2.20220009998ANTI-VEGF PROTEIN COMPOSITIONS AND METHODS FOR PRODUCING THE SAME
US 13.01.2022
Int.Class C07K 14/71
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
71for growth factors; for growth regulators
Appl.No 17460578 Applicant Regeneron Pharmaceuticals, Inc. Inventor Shunhai Wang

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

3.WO/2022/007205METHOD FOR INCREASING CONTENT OF INTRACELLULAR HEME IN ESCHERICHIA COLI
WO 13.01.2022
Int.Class C12N 1/21
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
Appl.No PCT/CN2020/118317 Applicant JIANGNAN UNIVERSITY Inventor TANG, Lei
A method for increasing the content of intracellular heme in escherichia coli, comprising: knocking out a gene mscS for encoding a small-conductivity mechanosensitive ion channel protein, knocking out a gene aroG for encoding 3-deoxy-arabinoheptulose-7 phosphate synthase, or overexpressing a gene hemA for encoding glutamyl-tRNA reductase in escherichia coli. The recombinant bacteria obtained by construction are cultured in an LB culture medium, and the content of the heme can reach 47.6 µmol·L-1.
4.20220010059POLYHYDROXYALKANOATES EXTRACTION SYSTEM
US 13.01.2022
Int.Class C08G 63/89
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
63Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
88Post-polymerisation treatment
89Recovery of the polymer
Appl.No 17026993 Applicant National Chi Nan University Inventor Yung-Pin TSAI

A polyhydroxyalkanoates extraction system comprises a pretreatment subsystem, an extraction subsystem and a recycling subsystem. The pretreatment subsystem comprises a fermentation device and an activation device so as to carry out a microorganism acclimation process. The extraction subsystem comprises a freezing device, a pretreatment device and an extraction device. The extraction subsystem is used for receiving a third sludge so that the third sludge is subjected to a freezing process, a pretreatment process, an extraction process and a purification process in the freezing device to form a polyhydroxyalkanoates mixture, and the extraction device performs a precipitation process to generate polyhydroxyalkanoates precipitate. The recycling subsystem comprises an aerobic sludge digestion device and a sequencing batch reactor activated sludge treatment device so as to carry out an aerobic sludge digestion process and a sequencing batch reactor activated sludge process.

5.WO/2022/008478PROCESS FOR PRODUCING SINGLE CELL PROTEIN
WO 13.01.2022
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/EP2021/068582 Applicant UNIBIO A/S Inventor NANDY, Subir, Kumar
The present invention relates to a process for providing a first reaction product by a first fermentation process conducted in a first Loop reactor, the method comprising the steps of: (i) adding an inoculum comprising one or more methanogenic microorganism to the first Loop reactor providing a first inoculated fermentation medium; (ii) adding a gaseous hydrogen (H2) to the first inoculated fermentation medium; (iii) adding a first carbon source to the first inoculated fermentation medium; (iv) allowing the first fermentation medium to ferment, providing the first reaction product; and (v) isolating the first reaction product provided in step (iv).
6.WO/2022/008628METHOD FOR PREPARATION OF 2,6-BIS(HYDROXYMETHYL)PYRIDINE VIA ENZYMATIC CATALYSIS
WO 13.01.2022
Int.Class C12P 13/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
Appl.No PCT/EP2021/068920 Applicant VIO CHEMICALS AG Inventor PANKE, Sven
The present invention relates to the provision of an enzymatic method for the preparation of 2, 6-bis(hydroxymethyl) pyridine (Formula I) using as substrate 2,6-Dimethlypyridine (2,6-lutidene) and the multicomponent xylene monooxygenase comprising XylM and XylA from Pseudomonas putida (Arthrobacter siderocapsulatus). The enzymatic method of the present invention is advantageous over conventional synthetic preparations, providing access to the title compound with a one-step enzymatic procedure.
7.20220010034METHOD AND DEVICE FOR THE DIGESTION OF STARCH
US 13.01.2022
Int.Class C08B 30/12
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
BPOLYSACCHARIDES; DERIVATIVES THEREOF
30Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
12Degraded or non-chemically modified starch; Bleaching of starch
Appl.No 17283905 Applicant PGA PUTZ-GRANITZER-ANLAGENBAU GESELLSCHAFT M.B.H. Inventor Christian STIRN

With a method for digesting starch, an aqueous slurry of the starch is treated with steam in a cooking vessel and in this case exposed to shear forces, wherein the starch-containing slurry is heated to a temperature of between 85□C and 110□C in the cooking vessel by introducing steam, and the digestion step is implemented until the desired degree of digestion has been reached. Also described is a cooking vessel that can be used when the method for digesting starch is being carried out.

8.20220010340BACTERIAL-DERIVED NITROGEN SOURCE FOR ETHANOL FERMENTATION
US 13.01.2022
Int.Class C12P 7/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
06Ethanol, i.e. non-beverage
Appl.No 17372291 Applicant Lallemand Hungary Liquidity Management LLC Inventor James L. Steele

The present disclosure concerns a recombinant bacterial host cell capable of providing a nitrogen source to a yeast during fermentation to make ethanol. The recombinant bacterial host cell is capable of converting a biomass into ethanol. The recombinant bacterial host cell has at least one first genetic modification. The at least one genetic modifications confers to the recombinant bacterial host cell the ability to increase, when compared to a corresponding control bacterial cell lacking the at least one first genetic modification, the proteolytic activity associated with the recombinant bacterial host cell. The at least one genetic modification also confers the recombinant bacterial host cell the ability to provide a nitrogen source to a yeast capable of converting the biomass into ethanol, wherein the nitrogen source comprises a peptide, an amino acid and/or ammonia.

9.WO/2022/007145GENE MODULE FOR EFFICIENTLY DEGRADING FEATHER AND SYNTHESIZING ARTIFICIAL HEMEPROTEIN, AND APPLICATION THEREOF
WO 13.01.2022
Int.Class C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
Appl.No PCT/CN2020/111685 Applicant BIOTECHNOLOGY RESEARCH INSTITUTE , CHINESE ACADEMY OF AGRICULTURAL SCIENCES Inventor LIN, Min
A functional module SyKerMb capable of efficiently degrading poultry feather waste and synthesizing a novel hemeprotein is artificially designed and constructed by using a synthetic biology method. A recombinant vector of the functional module is constructed, and assembled and expressed in chassis microorganism cell Escherichia coli. Experiments prove that engineering strain integrating the transformation function module has the biological synthesis capability of degrading animal feather waste and efficiently synthesizing an artificial hemeprotein, and can be applied in related industrial production of artificial protein preparations and future artificial food.
10.20220010263SUPERFLUIDS DISRUPTION OF SACCHAROMYCES CEREVISIAE (YEAST), CELL WALL DISINTEGRATION INTO NANOPARTICLES AND FRACTIONATION INTO BETA-GLUCANS, CHITIN AND MANNANS (MANNOPROTEINS)
US 13.01.2022
Int.Class C12N 1/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
06Lysis of microorganisms
Appl.No 17374966 Applicant Trevor Percival Castor Inventor Trevor Percival Castor

The present invention is directed to methods and apparatus for and products from disrupting, removing intracellular proteins, enzymes and nucleic acids, spray drying, lipid extraction, and making nanoparticles of Saccharomyces cerevisiae (yeast) cell wall followed by acid and/or enzymatic hydrolysis to produce Beta (β)-glucans, chitin and mannans (mannoproteins). The process and apparatus feature critical, supercritical, or near critical fluids for disruption of yeast and making yeast cell wall nanoparticles. The product materials retain full activity and are devoid of residual processing chemicals such as solvents, salts, or surfactants.