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1. WO2021023896 - PROBE-INDUCED HETERODUPLEX MOBILITY ASSAY

Publication Number WO/2021/023896
Publication Date 11.02.2021
International Application No. PCT/EP2020/072434
International Filing Date 10.08.2020
IPC
C12Q 1/6813 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
CPC
C12Q 1/6827
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6827for detection of mutation or polymorphism
Applicants
  • UNIVERSITÄT ZÜRICH [CH]/[CH]
  • PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY [JP]/[JP]
Inventors
  • KAKUI, Hiroyuki
  • SHIMIZU, Kentaro, K.
  • YAMAZAKI, Misako
Agents
  • JUNGHANS, Claas
Priority Data
19190891.208.08.2019EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) PROBE-INDUCED HETERODUPLEX MOBILITY ASSAY
(FR) DOSAGE DE MOBILITÉ HÉTÉRODUPLEX INDUIT PAR SONDE
Abstract
(EN) The present invention relates to a method for distinguishing a first nucleic acid sequence from a second nucleic acid sequence by electrophoresis. The first nucleic acid comprises a first common sequence tract, a variable sequence tract and a second common sequence tract and the second nucleic acid comprises a first common sequence tract, optionally an variable sequence tract and a second common sequence tract. The first and the second nucleic acid sequence is contacted with a probe sequence that is reverse complementary to the first and second common sequence tract under conditions allowing the hybridization of the probe sequence to the first and second nucleic acid sequence, thereby forming a first probe hybrid and a second probe hybrid. Subsequently, the first and second probe hybrids are submitted to electrophoresis to detect the electrophoretic mobility of the first and second probe hybrid.
(FR) La présente invention concerne un procédé pour distinguer une première séquence d'acides nucléiques d'une seconde séquence d'acides nucléiques par électrophorèse. Le premier acide nucléique comprend un premier tractus de séquence commun, un tractus de séquence variable et un second tractus de séquence commun et le second acide nucléique comprenant un premier tractus de séquence commun, éventuellement un tractus de séquence variable et un second tractus de séquence commun. La première et la seconde séquence d'acide nucléique sont mises en contact avec une séquence de sonde étant complémentaire à la première et à la seconde séquence de séquence commune dans des conditions permettant l'hybridation de la séquence de sonde à la première et à la seconde séquence d'acide nucléique, formant ainsi un premier hybride de sonde et un second hybride de sonde. Ensuite, les premier et second hybrides de sonde sont soumis à une électrophorèse pour détecter la mobilité électrophorétique des premier et second hybrides de sonde.
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