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1. WO2020026979 - MEMBRANE PROTEIN ACTIVITY MEASUREMENT METHOD

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Publication Number WO/2020/026979
Publication Date 06.02.2020
International Application No. PCT/JP2019/029424
International Filing Date 26.07.2019
IPC
C12N 15/63 2006.1
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09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C07K 14/705 2006.1
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07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
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5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
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C12Q 1/06 2006.1
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06Quantitative determination
C12Q 1/66 2006.1
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
66involving luciferase
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1086Preparation or screening of expression libraries, e.g. reporter assays
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09Recombinant DNA-technology
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79Vectors or expression systems specially adapted for eukaryotic hosts
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2830Vector systems having a special element relevant for transcription
001controllable enhancer/promoter combination
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06Quantitative determination
C12Q 1/66
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
66involving luciferase
Applicants
  • 国立大学法人東京大学 THE UNIVERSITY OF TOKYO [JP]/[JP]
Inventors
  • 池田　祐一 IKEDA Yuichi
  • 熊谷　英敏 KUMAGAI Hidetoshi
Agents
  • 小林　浩 KOBAYASHI Hiroshi
  • 大森　規雄 OHMORI Norio
  • 鈴木　康仁 SUZUKI Yasuhito
Priority Data
2018-14417131.07.2018JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) MEMBRANE PROTEIN ACTIVITY MEASUREMENT METHOD
(FR) MÉTHODE DE MESURE DE L'ACTIVITÉ DE PROTÉINES MEMBRANAIRES
(JA) 膜タンパク質活性測定法
front page image
Abstract
(EN) The present invention provides a comprehensive assay capable of comprehensively measuring interactors for various different types of proteins. The present invention provides a system for detecting the activation of a protein by integrating changes in the transcription regulatory region of a gene the expression of which is changed by activation of the protein via a plurality of factors. The stimulation response ratio of transcription activity before and after stimulation by ligands of each transcription regulatory region is synergistically integrated to permit construction of a highly sensitive reporter assay system.
(FR) La présente invention concerne une méthode d'analyse intégrée apte à mesurer de manière intégrée des interacteurs pour différents types de protéines variées. La présente invention concerne un système permettant de détecter l'activation d'une protéine en intégrant les changements de la région régulatrice de la transcription d'un gène dont l'expression est modifiée par l'activation de la protéine par l'intermédiaire d'une multiplicité de facteurs. Le rapport de la réponse de stimulation de l'activité de transcription avant et après la stimulation par des ligands de chaque région régulatrice de la transcription est intégré de manière synergique pour permettre la construction d'un système analytique rapporteur hautement sensible.
(JA) 本発明は、タンパク質の種々の異なる種類に対する相互作用因子を網羅的に測定することができる網羅的なアッセイを提供する。　本発明では、タンパク質の活性化によって発現が変化する遺伝子の転写調節領域における変化を複数の因子を介在させて統合することによって、当該タンパク質の活性化を検出する系が提供される。それぞれの転写調節領域が有するリガンド刺激前後での転写活性の刺激応答比が相乗的に統合され、高感度なレポーターアッセイ系が構築され得る。
Related patent documents
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