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1. (WO2015034012) NEW METHOD FOR INDUCING DOPAMINE-PRODUCING NEURAL PRECURSOR CELLS
Latest bibliographic data on file with the International Bureau
Pub. No.: WO/2015/034012 International Application No.: PCT/JP2014/073372
Publication Date: 12.03.2015 International Filing Date: 04.09.2014
IPC:
C12N 5/0797 (2010.01) ,A61K 35/30 (2015.01) ,A61P 25/16 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
079
Neural cells
0797
Stem cells; Progenitor cells
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
30
Nerves; Brain
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
25
Drugs for disorders of the nervous system
14
for treating abnormal movements, e.g. chorea, dyskinesia
16
Anti-Parkinson drugs
Applicants:
国立大学法人京都大学 KYOTO UNIVERSITY [JP/JP]; 京都府京都市左京区吉田本町３６番地１ 36-1, Yoshida-honmachi, Sakyo-ku, Kyoto-shi, Kyoto 6068501, JP
国立大学法人大阪大学 OSAKA UNIVERSITY [JP/JP]; 大阪府吹田市山田丘１番１号 1-1, Yamadaoka, Suita-shi, Osaka 5650871, JP (AL, AT, BA, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, JP, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR, US)
エーザイ・アール・アンド・ディー・マネジメント株式会社 EISAI R&D MANAGEMENT CO., LTD. [JP/JP]; 東京都文京区小石川四丁目６番１０号 4-6-10, Koishikawa, Bunkyo-ku, Tokyo 1128088, JP (AL, AT, AU, BA, BE, BG, CA, CH, CN, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, JP, KR, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SG, SI, SK, SM, TR, US)
Inventors:
高橋　淳 TAKAHASHI, Jun; JP
土井　大輔 DOI, Daisuke; JP
佐俣　文平 SAMATA, Bumpei; JP
関口　清俊 SEKIGUCHI, Kiyotoshi; JP
尾野　雄一 ONO, Yuichi; JP
Agent:
川口　嘉之 KAWAGUCHI, Yoshiyuki; JP
Priority Data:
2013-18438705.09.2013JP
Title (EN) NEW METHOD FOR INDUCING DOPAMINE-PRODUCING NEURAL PRECURSOR CELLS
(FR) NOUVEAU PROCÉDÉ POUR L'INDUCTION DE CELLULES PRÉCURSEURS NEURALES PRODUISANT DE LA DOPAMINE
(JA) 新規ドーパミン産生神経前駆細胞の誘導方法
Abstract:
(EN) Provided is a method for manufacturing dopamine-producing neural precursor cells from pluripotent stem cells, the method comprising: (i) a step in which pluripotent stem cells are cultured adherently on an extracellular matrix in a culture solution containing a reagent selected from the group consisting of BMP inhibitors, TGFβ inhibitors, SHH signal stimulants, and FGF8 and GSK3β inhibitors; (ii) a step in which a substance than binds to Corin and/or a substance that binds to Lrtm1 is used to collect Corin and/or Lrtm1 positive cells from the cells obtained in step (i); and (iii) a step in which the cells obtained in step (ii) are cultured in suspension in a culture solution containing a neurotrophic factor.
(FR) L'invention porte sur un procédé pour la production de cellules précurseurs neurales produisant de la dopamine à partir de cellules souches pluripotentes, le procédé comprenant : (i) une étape dans laquelle des cellules souches pluripotentes sont cultivées de façon adhérente sur une matrice extracellulaire dans une solution de culture contenant un réactif choisi dans le groupe constitué par les inhibiteurs de BMP, les inhibiteurs de TGFβ, les stimulants du signal SHH et les inhibiteurs de FGF8 et de GSK3β ; (ii) une étape dans laquelle une substance qui se lie à la corine et/ou une substance qui se lie à Lrtm1 est utilisée pour recueillir des cellules positives à la corine et/ou à Lrtm1 à partir des cellules obtenues dans l'étape (i) ; et (iii) et une étape dans laquelle les cellules obtenues dans l'étape (ii) sont cultivées en suspension dans une solution de culture contenant un facteur neurotrophique.
(JA) （i）多能性幹細胞をBMP阻害剤、TGFβ阻害剤、SHHシグナル刺激剤、FGF8およびGSK3β阻害剤から成る群より選択される試薬を含む培養液中で細胞外基質上にて接着培養する工程、 （ii）前記工程(i)で得られた細胞からCorinに結合する物質および/またはLrtm1に結合する物質を用いてCorinおよび／またはLrtm1陽性細胞を収集する工程、および （iii）前記工程（ii）で得られた細胞を神経栄養因子を含む培養液中で浮遊培養する工程を含む、多能性幹細胞からドーパミン産生神経前駆細胞を製造する方法を提供する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
CA2923592SG11201601720REP3042951US20160215260KR1020160053936CN105849255
AU2014316100MYPI 2016700764