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1. EP3913069 - METHOD AND DIAGNOSTIC KIT FOR MULTIPLE DETECTION OF VIRUSES OF THE FAMILY CORONAVIRIDAE: SARS-COV-2, SARS-COV, HCOV AND MERS-COV

Office
European Patent Office
Application Number 20197796
Application Date 23.09.2020
Publication Number 3913069
Publication Date 24.11.2021
Publication Kind A4
IPC
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
CPC
C12Q 1/701
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
C12Q 2600/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
16Primer sets for multiplex assays
Applicants UNIV DEGLI STUDI DI BARI ALDO MORO
UNIV PHAN CHAU TRINH
Inventors INCHINGOLO FRANCESCO
BALLINI ANDREA
ISACCO GARGIULO CIRO
DIPALMA GIANNA
INCHINGOLO ALESSIO DANILO
INCHINGOLO ANGELO MICHELE
DIEM KIEU NGUEYN CAO
HUNG VAN PHAM
Designated States
Priority Data 202000011701 20.05.2020 IT
Title
(DE) VERFAHREN UND DIAGNOSTISCHES KIT ZUM MEHRFACHNACHWEIS VON VIREN DER FAMILIE CORONAVIRIDAE: SARS-COV-2, SARS-COV, HCOV UND MERS-COV
(EN) METHOD AND DIAGNOSTIC KIT FOR MULTIPLE DETECTION OF VIRUSES OF THE FAMILY CORONAVIRIDAE: SARS-COV-2, SARS-COV, HCOV AND MERS-COV
(FR) PROCÉDÉ ET KIT DE DIAGNOSTIC POUR LA DÉTECTION MULTIPLE DE VIRUS DE LA FAMILLE CORONAVIRIDAE : SARS-COV-2, SARS-COV, HCOV ET MERS-COV
Abstract
(EN) The invention relates to a diagnostic kit for multiple detection of 4 viruses of the Family Coronaviridae: HCoV, SARS-CoV, MERS-CoV and the SARS-CoV-2 viral strain that has caused a pandemic of the disease known as COVID-19. The kit uses a "One-Step" approach with quantitative gene amplification after backward transcription of the viral genome (rRT-PCR).In order to avoid potential false negatives, the invention contains a double control using Porcine Epidemic Diarrhoea Virus (PEDV -CoV) and Ribonuclease P (RNase P-RP).