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1. WO2021145700 - CELLS HAVING HIGH ADAPTABILITY UNDER HYPOXIC CONDITIONS, AND USE THEREOF

Publication Number WO/2021/145700
Publication Date 22.07.2021
International Application No. PCT/KR2021/000546
International Filing Date 14.01.2021
IPC
C12N 5/0775 2010.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775Mesenchymal stem cells; Adipose-tissue derived stem cells
C12N 9/22 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
22Ribonucleases
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/11 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C07K 14/47 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
Applicants
  • 주식회사 툴젠 TOOLGEN INCORPORATED [KR]/[KR]
Inventors
  • 신은지 SHIN, Eun Ji
  • 이강인 LEE, Kang In
  • 이재영 LEE, Jae Young
Agents
  • 특허법인 아이피에스 IPS PATENT FIRM
Priority Data
10-2020-000461414.01.2020KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) CELLS HAVING HIGH ADAPTABILITY UNDER HYPOXIC CONDITIONS, AND USE THEREOF
(FR) CELLULES AYANT UNE ADAPTABILITÉ ÉLEVÉE DANS DES CONDITIONS HYPOXIQUES, ET LEUR UTILISATION
(KO) 저산소 환경 하에서 높은 적응력을 가지는 세포 및 이의 용도
Abstract
(EN)
The present application relates to cells having high adaptability under hypoxic conditions, and a preparation method therefor. Particularly, the present specification provides cells having high adaptability under hypoxic conditions, comprising at least one engineered gene having indels within a wild-type gene selected from the group consisting of HIF1AN, HIF3A, PHD2, TLR4 and PAI1. In addition, the present specification provides, as a method for preparing cells having high adaptability under hypoxic conditions, a gene editing method comprising introducing a CRISPR/Cas9 system into cells.
(FR)
La présente invention concerne des cellules ayant une adaptabilité élevée dans des conditions hypoxiques et un procédé de préparation de celles-ci. En particulier, la présente invention concerne des cellules ayant une adaptabilité élevée dans des conditions hypoxiques, comprenant au moins un gène modifié comportant des indels dans un gène de type sauvage choisi dans le groupe constitué de HIF1AN, HIF3A, PHD2, TLR4 et PAI1. De plus, la présente invention concerne, en tant que procédé de préparation de cellules ayant une adaptabilité élevée dans des conditions hypoxiques, un procédé d’édition de gène comprenant l’introduction d’un système CRISPR/Cas9 dans des cellules.
(KO)
본 출원은 저산소 조건에서 높은 적응력을 갖는 세포 및 이의 제조 방법에 관한 것이다. 구체적으로, 본 명세서에서는 HIF1AN, HIF3A, PHD2, TLR4 및 PAI1로 이뤄진 군에서 선택된 야생형 유전자 내에 인델을 가지는, 적어도 하나의 엔지니어링 된 유전자를 포함하는, 저산소 조건에서 높은 적응력을 갖는 세포를 제공한다. 추가적으로, 본 명세서에서는 상기 저산소 조건에서 높은 적응력을 갖는 세포를 제조하는 방법으로, CRISPR/Cas9 시스템을 세포 내에 도입하는 것을 포함하는 유전자 편집 방법을 제공한다.
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