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1. WO2022160049 - PROTECTION OF LINEAR DEOXYRIBONUCLEIC ACID FROM EXONUCLEOLYTIC DEGRADATION

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Publication Number WO/2022/160049
Publication Date 04.08.2022
International Application No. PCT/CA2022/050113
International Filing Date 27.01.2022
IPC
C12N 15/113 2010.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N 1/21 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/11 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12N 15/63 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12P 21/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
Applicants
  • THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO [CA]/[CA]
Inventors
  • PARDEE, Keith
  • NOROUZI, Masoud
Agents
  • KRUPNIK, Eduardo
Priority Data
63/142,09727.01.2021US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) PROTECTION OF LINEAR DEOXYRIBONUCLEIC ACID FROM EXONUCLEOLYTIC DEGRADATION
(FR) PROTECTION DE L'ACIDE DÉSOXYRIBONUCLÉIQUE LINÉAIRE CONTRE UNE DÉGRADATION EXONUCLÉOLYTIQUE
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Abstract
(EN) A linear double stranded deoxyribonucleic acid (dsDNA) molecule comprising operatively linked in the 5' to 3' direction: a) one or more Ter sites at the 5' terminus ("5' Ter"); b) a segment comprising a DNA sequence of interest; and c) one or more Ter sites at the 3' terminus ("3' Ter). Also methods of protecting DNA sequences of interest from exonuclease degradation using the DNA constructs of the present disclosure, cells transformed with the DNA construct of the present disclosure and cell-free synthetic biology system comprising a linear dsDNA molecule of the present disclosure.
(FR) L'invention concerne une molécule d'acide désoxyribonucléique bicaténaire (ADNds) linéaire comprenant de manière fonctionnelle et liés dans la direction 5' à 3' : a) un ou plusieurs sites Ter à l'extrémité 5' ("5'Ter") ; b) un segment comprenant une séquence d'ADN d'intérêt ; et c) un ou plusieurs sites Ter à l'extrémité 3' (" 3'Ter). L'invention concerne également des procédés de protection de séquences d'ADN d'intérêt contre une dégradation par exonucléase à l'aide des constructions d'ADN de la présente invention, des cellules transformées à l'aide de la construction d'ADN de la présente invention et un système de biologie synthétique acellulaire comprenant une molécule d'ADN bicaténaire linéaire de la présente invention.
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