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1. WO2021250274 - DEVICE AND METHOD FOR CAPTURING AND ANALYSING AIRBORNE ORGANISMS

Publication Number WO/2021/250274
Publication Date 16.12.2021
International Application No. PCT/EP2021/065932
International Filing Date 14.06.2021
IPC
C12Q 1/70 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
C12Q 1/04 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/24 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganism
C12Q 1/6888 2018.1
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1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
C12M 1/12 2006.1
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MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
12with sterilisation, filtration, or dialysis means
G01N 33/497 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
497of gaseous biological material, e.g. breath
CPC
C12M 37/00
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
37Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
C12Q 1/04
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QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/24
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
C12Q 1/6806
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6888
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
Applicants
  • CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC) [ES]/[ES]
Inventors
  • ALCAMI PERTEJO, Antonio
  • RASTROJO LASTRAS, Alberto
Agents
  • PONS ARIÑO, Angel
Priority Data
20382510.412.06.2020EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) DEVICE AND METHOD FOR CAPTURING AND ANALYSING AIRBORNE ORGANISMS
(FR) DISPOSITIF ET PROCÉDÉ DE CAPTURE ET D'ANALYSE D'ORGANISMES EN SUSPENSION DANS L'AIR
Abstract
(EN) The present invention refers to a device comprising polytetrafluorethylene (PTFE) filters and the use of the same for collecting, detecting and identifying organisms present in air ecosystems. The invention also provides a method suitable for the capture, detection and identification of whole airborne biological particles, including viruses and other important air pathogens, which involves the use of the device. This method allows toperform organism, preferably viral, metagenomics to sequence all DNA and RNA organisms captured in the filters. This methodology may be used to detect, for instance, SARS-CoV2 particles in air samples.
(FR) La présente invention concerne un dispositif comprenant des filtres en polytétrafluoréthylène (PTFE) et son utilisation pour collecter, détecter et identifier des organismes présents dans des écosystèmes aériens. L'invention fournit également un procédé adapté à la capture, à la détection et à l'identification de particules biologiques entières en suspension dans l'air, y compris des virus et d'autres agents pathogènes aériens importants, impliquant l'utilisation du dispositif. Ce procédé permet de réaliser une métagénomique des organismes, de préférence viraux, afin de séquencer tous les organismes à ADN et ARN capturés dans les filtres. Cette méthodologie peut être utilisée pour détecter, par exemple, des particules de SRAS-CoV2 dans des échantillons d'air.
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