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The invention discloses an assembly type negative pressure isolation chamber, in which the necessary configuration of the negative pressure isolation ward is contained in a hexahedral chamber. The chamber forms an independent building assembly unit, which can be integrated into or removed from the building system. The chamber can also be disassembled and transported, and the switching of use functions can be realized through the flexible combination and simple modification of components. The indoor wall of the chamber is light Clean and washable materials are integrated with furniture. The air conditioning system adopts internal circulation return air and independent temperature regulating device. The sewage pre disinfection link is added to the sewage system. The negative pressure isolation chamber can help the medical isolation building system expand rapidly in case of respiratory infectious disease outbreak and can carry out space mobility with the transfer of the epidemic situation.