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1. WO2022136477 - METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR WITH THE USE OF LARGE STOKES SHIFT FLUORESCENT DYES

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Publication Number WO/2022/136477
Publication Date 30.06.2022
International Application No. PCT/EP2021/087165
International Filing Date 21.12.2021
IPC
C12Q 1/686 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction
C12Q 1/6818 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
CPC
C12Q 1/6818
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
Applicants
  • F. HOFFMANN-LA ROCHE AG [CH]/[CH] (AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BE, BF, BG, BH, BJ, BN, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CL, CM, CN, CO, CR, CU, CY, CZ, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IR, IS, IT, JO, JP, KE, KG, KH, KM, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, ME, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SI, SK, SL, SM, SN, ST, SV, SY, SZ, TD, TG, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, WS, ZA, ZM, ZW)
  • ROCHE DIAGNOSTICS GMBH [DE]/[DE] (DE)
  • ROCHE MOLECULAR SYSTEMS, INC. [US]/[US] (US)
Inventors
  • NIERTH, Alexander
  • WANG, Jin
  • WANG, Fangnian
Agents
  • HÖVELMANN, Sascha
Priority Data
63/129,42322.12.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR WITH THE USE OF LARGE STOKES SHIFT FLUORESCENT DYES
(FR) PROCÉDÉS POUR RÉALISER UNE PCR MULTIPLEX EN TEMPS RÉEL AVEC UTILISATION DE COLORANTS FLUORESCENTS À GRAND DÉPLACEMENT DE STOKES
Abstract
(EN) The present invention allows for the expansion of multiplexing capabilities of common PCR devices by using fluorogenic PCR probes made of large Stokes shift (LSS) fluorescent dyes. With this approach, no changes of the hardware or software components in the instrument are required.
(FR) La présente invention permet l'expansion de capacités de multiplexage de dispositifs PCR communs au moyen de sondes PCR fluorogéniques constituées de colorants fluorescents à grand déplacement de Stokes (LSS). Cette approche permet de ne pas apporter de changements aux composants matériels ou logiciels dans l'instrument utilisé.
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