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1. WO2022135246 - R GENE FOR CONTROLLING MATCHING OF SOYBEAN-RHIZOBIUM, PROTEIN AND USE THEREOF

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Publication Number WO/2022/135246
Publication Date 30.06.2022
International Application No. PCT/CN2021/138591
International Filing Date 16.12.2021
IPC
C12N 15/29 2006.1
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29Genes encoding plant proteins, e.g. thaumatin
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113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
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Applicants
  • 河南大学 HENAN UNIVERSITY [CN]/[CN]
  • 华中农业大学 HUAZHONG AGRICULTURAL UNIVERSITY [CN]/[CN]
Inventors
  • 王学路 WANG, Xuelu
  • 张豹 ZHANG, Bao
  • 李友国 LI, Youguo
Agents
  • 郑州优盾知识产权代理有限公司 ZHENGZHOU YOUDUN INTELLCTUAL PROPERTY AGENCY CO., LTD.
Priority Data
202011537197.423.12.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) R GENE FOR CONTROLLING MATCHING OF SOYBEAN-RHIZOBIUM, PROTEIN AND USE THEREOF
(FR) GÈNE R POUR COMMANDER L'APPARIEMENT DU SOJA-RHIZOBIUM, PROTÉINE ET SON UTILISATION
(ZH) 一种控制大豆-根瘤菌匹配性的R基因及其蛋白质和应用
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Abstract
(EN) An R gene for controlling the soybean-rhizobium matching performance, and a protein and the use thereof. The GmNNL1 genome sequence of the gene GmNNL1 disclosed in the present invention in the soybean line Hengfeng Wudou is as shown in SEQ ID NO. 2, and the encoded amino acid sequence is as shown in SEQ ID NO. 3. The R gene GmNNL1 of the soybean is an effective gene which is capable of regulating and controlling the number of nodulations of a specific rhizobium on the soybean, and can regulate and control the number of nodulations by directly identifying the haplotype of the specific effector protein NopP of the slow-growing rhizobium, and limit the symbiotic nodulations of the indigenous rhizobium, such that the soybean preferentially nodulates with an artificially applied high-efficiency rhizobium inoculant applied by a human, and the symbiotic nitrogen fixation capability can be improved.
(FR) L'invention concerne également un gène R permettant de réguler les performances d'appariement de rhizobium de soja, et une protéine Et son utilisation. La séquence du génome GmNNL1 du Gène GmNNL1 décrite dans la présente invention dans la lignée de Soja Hengfeng Wudou est Telle que Représentée Dans SEQ ID NO 2, Et la séquence d'acides aminés codée est Telle Que Représentée dans SEQ ID NO 3. Le gène R GmNNL1 du soja est un gène efficace qui est capable de réguler et de réguler le nombre de nodulations d'un rhizobium spécifique sur le soja, et peut réguler et réguler le nombre de nodulations par identification directe de l'haplotype de la protéine Effectrice spécifique NopP du rhizobium à croissance lente, et limiter les nodulations symbiotiques du rhizobium indigène, de telle sorte que le soja nodulise de manière préférentielle avec un inoculant de rhizobium à efficacité élevée appliqué artificiellement appliqué par un être humain, et la capacité de fixation d'azote symbiotique peut être améliorée.
(ZH) 一种控制大豆-根瘤菌匹配性的R基因及其蛋白质和应用。本发明公开的基因GmNNL1在大豆品系横峰乌豆中的GmNNL1基因组序列为SEQ ID NO.2所示，编码的氨基酸序列为SEQ ID NO.3所示。大豆的R基因GmNNL1是一个可以调控特定根瘤菌在大豆上结瘤数量的有效基因，它可以通过直接识别慢生型根瘤菌特定效应蛋白NopP的单倍型来调控结瘤数量，限制土著根瘤菌的共生结瘤，使得大豆优先与人为施加的高效根瘤菌剂结瘤，并且能够提高共生固氮能力。
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