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1. WO2022121301 - PROKARYOTE-DERIVED ARGONAUTE PROTEIN AND APPLICATION THEREOF

Publication Number WO/2022/121301
Publication Date 16.06.2022
International Application No. PCT/CN2021/105421
International Filing Date 09.07.2021
IPC
C12N 9/22 2006.1
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
22Ribonucleases
C12N 15/55 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
55Hydrolases (3)
C12P 19/34 2006.1
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PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 15/63 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/867 2006.1
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
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C12N 15/63
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/86
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2740/10043
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2740Reverse Transcribing RNA Viruses
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2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
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C12N 9/22
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
22Ribonucleases ; RNAses, DNAses
C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applicants
  • 湖北大学 HUBEI UNIVERSITY [CN]/[CN]
Inventors
  • 马立新 MA, Lixin
  • 王亚平 WANG, Yaping
  • 刘洋 LIU, Yang
  • 姜小满 JIANG, Xiaoman
Agents
  • 武汉智嘉联合知识产权代理事务所(普通合伙) WUHAN ZHI JIA JOINT INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP)
Priority Data
202011457521.111.12.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) PROKARYOTE-DERIVED ARGONAUTE PROTEIN AND APPLICATION THEREOF
(FR) PROTÉINE ARGONAUTE DÉRIVÉE DE PROCARYOTE ET APPLICATION ASSOCIÉE
(ZH) 一种原核生物来源的Argonaute蛋白及其应用
Abstract
(EN) Provided are a mesophilic prokaryote kurthia massiliensis-derived Argonaute protein KmAgo and an application thereof. Also provided are a polynucleotide encoding the KmAgo and an expression vector containing the polynucleotide, a kit containing the KmAgo, a method for in vitro and intracellular cleavage of target RNA by using the KmAgo, and a method for site-specific modification of a genetic material of a cell.
(FR) La présente invention concerne une protéine Argonaute KmAgo dérivée du procaryote mésophile Kurthia massiliensis et une application associée. Sont également présents un polynucléotide codant pour la KmAgo et un vecteur d'expression contenant le polynucléotide, un kit contenant la KmAgo, un procédé de clivage in vitro et intracellulaire de l'ARN cible en utilisant la KmAgo, et un procédé de modification à site spécifique d'un matériel génétique d'une cellule.
(ZH) 提供了一种来源于中温原核生物kurthia massiliensis的Argonaute蛋白KmAgo及其应用。还提供了编码所述KmAgo的多核苷酸和包含所述多核苷酸的表达载体,包含所述KmAgo的试剂盒,以及利用所述KmAgo进行体外、细胞内切割靶RNA的方法,和位点特异性修饰细胞的遗传材料的方法。
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