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1. WO2022135173 - ESCHERICHIA COLI STRAIN AND USE THEREOF IN PRODUCTION OF POLYSIALIC ACID

Publication Number WO/2022/135173
Publication Date 30.06.2022
International Application No. PCT/CN2021/136947
International Filing Date 10.12.2021
IPC
C12N 1/20 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
C12P 19/26 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
C07H 1/00 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1Processes for the preparation of sugar derivatives
C07H 1/06 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1Processes for the preparation of sugar derivatives
06Separation; Purification
C07H 7/033 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
7Compounds containing non-saccharide radicals linked to saccharide radicals by a carbon-to-carbon bond
02Acyclic radicals
033Uronic acids
C12R 1/19 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
185Escherichia
19Escherichia coli
CPC
C07H 1/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1Processes for the preparation of sugar derivatives
C07H 1/06
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1Processes for the preparation of sugar derivatives
06Separation; Purification
C07H 7/033
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
7Compounds containing non-saccharide radicals linked to saccharide radicals by a carbon-to-carbon bond
02Acyclic radicals
033Uronic acids
C12P 19/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
Applicants
  • 华熙生物科技股份有限公司 BLOOMAGE BIOTECHNOLOGY CORPORATION LIMITED [CN]/[CN]
Inventors
  • 张天萌 ZHANG, Tianmeng
  • 刘英杰 LIU, Yingjie
  • 戚明 QI, Ming
  • 刘金钊 LIU, Jinzhao
  • 王景 WANG, Jing
Agents
  • 北京唐颂永信知识产权代理有限公司 BEIJING TANGSONG IP FIRM
Priority Data
202011559576.325.12.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) ESCHERICHIA COLI STRAIN AND USE THEREOF IN PRODUCTION OF POLYSIALIC ACID
(FR) SOUCHE ESCHERICHIA COLI ET SON UTILISATION DANS LA PRODUCTION D'ACIDE POLYSIALIQUE
(ZH) 一株大肠埃希氏菌及其在产聚唾液酸中的应用
Abstract
(EN) Provided is an Escherichia coli strain, H03A2190830. The deposit number thereof is CCTCC NO: M2019900. Further provided are a method for producing a polysialic acid by means of using the Escherichia coli, and a method for producing a sialic acid by means of using the polysialic acid prepared from the present method. When using the strain to produce the polysialic acid, not only can the production time be shortened and the production cost be reduced, but also a polysialic acid with a relatively high yield can be obtained, and therefore the strain has good industrial application prospects.
(FR) La présente invention concerne une souche d'Escherichia coli, H03A2190830. Son numéro de dépôt est CCTCC NO : M2019900. L'invention concerne en outre un procédé de production d'un acide polysialique au moyen de l'Escherichia coli, et un procédé de production d'un acide sialique au moyen de l'acide polysialique préparé à partir du présent procédé. En utilisant la souche pour produire l'acide polysialique, on peut non seulement raccourcir le temps de production et réduire le coût de production, mais aussi obtenir un acide polysialique avec un rendement relativement élevé, et donc la souche a de bonnes perspectives d'application industrielle.
(ZH) 提供了一株大肠埃希氏菌H03A2190830,该菌株的保藏号为CCTCC NO:M2019900。还提供了使用该大肠埃希氏菌生产聚唾液酸的方法,以及使用该方法制得的聚唾液酸生产唾液酸的方法。采用该菌株生产聚唾液酸时,不仅能缩短生产时间,降低生产成本,还能得到较高产量的聚唾液酸,具有很好的工业应用前景。
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