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1. WO2021195277 - MULTIPLEXED METHODS FOR DETECTING TARGET RNAS

Publication Number WO/2021/195277
Publication Date 30.09.2021
International Application No. PCT/US2021/023978
International Filing Date 24.03.2021
IPC
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C40B 20/04 2006.1
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
20Methods specially adapted for identifying library members
04Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C12Q 1/6869 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
C40B 20/04
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
20Methods specially adapted for identifying library members
04Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
Applicants
  • PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US]/[US]
Inventors
  • KIRSCHNER, Marc W.
  • DAI, Mingjie
  • CHURCH, George M.
Agents
  • FITZGERALD, Mark J.
  • BENN, Susanna C.
  • BRAICH, Ravinderjit S.
  • CROSBY, David F.
  • EISENSTEIN, Ronald I.
  • HUFF, Shayne Y.
  • KLING, Nicole D.
  • PTASHKA, Teresa A.
  • RESNICK, David S.
  • DE REGE THESAURO, Francesco
Priority Data
62/994,07224.03.2020US
63/040,79018.06.2020US
63/159,03310.03.2021US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) MULTIPLEXED METHODS FOR DETECTING TARGET RNAS
(FR) PROCÉDÉS MULTIPLEXÉS POUR LA DÉTECTION D'ARN CIBLES
Abstract
(EN) The technology described herein is directed to methods, kits, compositions, and systems for detecting a target RNA, such as a small amount of viral RNA. In one aspect, described herein are methods of detecting the target RNA, using primers comprising at least one barcode region. In other aspects, described herein are kits, compositions, and systems suitable to practice the methods described herein to detect the target RNA.
(FR) La technologie décrite dans la présente description concerne des procédés, des kits, des compositions et des systèmes de détection d'un ARN cible, tel qu'une petite quantité d'ARN viral. Selon un aspect, l'invention concerne des procédés de détection de l'ARN cible, à l'aide d'amorces comprenant au moins une région de code à barres. Selon d'autres aspects, l'invention concerne des kits, des compositions et des systèmes appropriés pour mettre en œuvre les procédés décrits dans la description pour détecter l'ARN cible.
Latest bibliographic data on file with the International Bureau