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1. WO2022147868 - SEWAGE SURVEILLANCE FOR SARS-COV-2

Publication Number WO/2022/147868
Publication Date 14.07.2022
International Application No. PCT/CN2021/074675
International Filing Date 01.02.2021
Chapter 2 Demand Filed 15.08.2022
IPC
C12Q 1/6806 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
G01N 33/18 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
18Water
C12Q 1/70 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
G01N 33/569 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6851
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6851Quantitative amplification
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
G01N 1/10
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
10in the liquid or fluent state
G01N 1/4077
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
40Concentrating samples
4077by other techniques involving separation of suspended solids
G01N 2001/1043
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
02Devices for withdrawing samples
10in the liquid or fluent state
1031Sampling from special places
1043from sewers
Applicants
  • THE UNIVERSITY OF HONG KONG [CN]/[CN]
Inventors
  • ZHANG, Tong
  • DENG, Yu
  • ZHENG, Xiawan
  • XU, Xiaoqing
  • POON, Leo
  • TUN, Hein Min
Agents
  • CHINA PATENT AGENT (H.K.) LTD.
Priority Data
63/135,26208.01.2021US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SEWAGE SURVEILLANCE FOR SARS-COV-2
(FR) SURVEILLANCE DES EAUX D'ÉGOUT POUR SARS-COV-2
Abstract
(EN) Systems for sensitive and accurate detection ofinfectious agents in samples from sewage and wastewater have been established. The systems can rapidly and effectively detect and record the presence ofSARS-CoV-2 and other viruses within wastewater and sewage from specific locations. The systems employ Quantitative Polymerase Chain Reaction (RT-qPCR) to identify and quantify nucleic acids indicative ofSARS-CoV-2 and other viruses within samples obtained from sewagesheds. The systems reproducibly and accurately identify and quantitate viral RNAs for enable rapid and early monitoring ofmunicipal water systems in areas housing or related to patients infected with SARS-CoV-2 and/or other viruses, and provide databases to monitor the presence ofinfectious agents within sewagesheds on a community-wide level.
(FR) L'invention concerne des systèmes pour la détection sensible et précise d'agents infectieux dans des échantillons à partir des eaux d'égout et des eaux usées ayant été établis. Les systèmes peuvent détecter et enregistrer rapidement et efficacement la présence de SARS-CoV-2 et d'autres virus dans les eaux d'égout et les eaux usées à partir d'emplacements spécifiques. Les systèmes utilisent une réaction quantitative en chaîne par polymérase (RT-qPCR) pour identifier et quantifier des acides nucléiques indicatifs du SARS-CoV-2 et d'autres virus dans des échantillons obtenus à partir de hangars d'assainissement. Les systèmes identifient et quantifient de manière reproductible et précise des ARN viraux pour permettre une surveillance rapide et précoce de systèmes d'eau municipaux dans des zones comportant ou associées à des patients infectés par le SARS-CoV-2 et/ou d'autres virus et fournissent des bases de données pour surveiller la présence d'agents infectieux à l'intérieur de hangars d'assainissement sur un niveau communautaire.
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