WO2022139522 GLYCOSYLTRANSFERASE AND STEVIOL GLUCOSIDE PREPARATION METHOD USING SAME

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Publication Number WO/2022/139522

Publication Date 30.06.2022

International Application No. PCT/KR2021/019767

International Filing Date 23.12.2021

IPC

CPC

Applicants

주식회사 삼양사 SAMYANG CORPORATION [KR]/[KR]

Inventors

김정민 KIM, Jeong Min
고혁진 KO, Hyeok-Jin
문준호 MOON, Jun-Ho
최은수 CHOI, Eunsoo

Agents

팬코리아특허법인 PANKOREA PATENT AND LAW FIRM

Priority Data

10-2020-0182517

23.12.2020

Publication Language Korean (ko)

Filing Language Korean (KO)

Designated States

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Title

(EN) GLYCOSYLTRANSFERASE AND STEVIOL GLUCOSIDE PREPARATION METHOD USING SAME

(FR) GLYCOSYLTRANSFÉRASE ET PROCÉDÉ DE PRÉPARATION DE GLUCOSIDE DE STÉVIOL L'UTILISANT

(KO) 당전이 효소 및 이를 이용한 스테비올 배당체의 제조방법

Abstract

(EN) The present invention relates to a glycosyltransferase, a composition for producing a steviol glucoside by using same, and a preparation method therefor, and, more specifically, to: a rebaudioside glycosyltransferase for transferring glucose to a steviol glucoside; a recombinant strain expressing the enzyme; and a method for producing rebaudioside, which is steviol glucoside, by using the enzyme and strain.

(FR) La présente invention concerne une glycosyltransférase, une composition pour la production d'un glucoside de stéviol à l'aide de celle-ci, et son procédé de préparation, et, plus spécifiquement : une rébaudioside glycosyltransférase pour transférer du glucose à un glucoside de stéviol; une souche recombinante exprimant l'enzyme; et un procédé de production de rébaudioside, qui est du glucoside de stéviol, à l'aide de l'enzyme et de la souche.

(KO) 본 발명은 당전이 효소 및 이를 이용한 스테비올 배당체의 생산용 조성물 및제조방법에 관한 것으로서, 더욱 자세하게는 스테비올 배당체에 포도당을 전이하는 레바우디오사이드의 당전이 효소, 상기 효소를 발현하는 재조합 균주, 및 이들을 이용한 스테비올 배당체인 레바우디오사이드 생산방법에 관한 것이다.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10	Transferases (2.)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
80	for fungi
81	for yeasts

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19	Preparation of compounds containing saccharide radicals
44	Preparation of O-glycosides, e.g. glucosides
56	having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10	Transferases (2.)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
80	for fungi
81	for yeasts

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1. WO2022139522 - GLYCOSYLTRANSFERASE AND STEVIOL GLUCOSIDE PREPARATION METHOD USING SAME