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1. WO2022136207 - IMPROVED MEANS AND METHODS FOR PRODUCING ISOBUTENE FROM 3-METHYLCROTONIC ACID

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Publication Number WO/2022/136207
Publication Date 30.06.2022
International Application No. PCT/EP2021/086687
International Filing Date 20.12.2021
IPC
C12P 5/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
5Preparation of hydrocarbons
02acyclic
C12P 7/40 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group
C12N 9/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
C12N 9/88 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88Lyases (4.)
CPC
C12N 9/1085
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1085transferring alkyl or aryl groups other than methyl groups (2.5)
C12N 9/88
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12P 5/026
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
5Preparation of hydrocarbons ; or halogenated hydrocarbons
02acyclic
026Unsaturated compounds, i.e. alkenes, alkynes or allenes
C12P 7/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group ; including Peroxycarboxylic acids
C12Y 205/01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
205Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
01transferring alkyl or aryl groups, other than methyl groups (2.5.1)
Applicants
  • GLOBAL BIOENERGIES [FR]/[FR]
Inventors
  • BENSOUSSAN, Claude
  • OLLIVIER, Frédéric
  • CHAYOT, Romain
  • THIBAUT, Denis
  • DELCOURT, Marc
Agents
  • VOSSIUS & PARTNER (NO 31)
Priority Data
20 215 872.121.12.2020EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) IMPROVED MEANS AND METHODS FOR PRODUCING ISOBUTENE FROM 3-METHYLCROTONIC ACID
(FR) MOYENS ET PROCÉDÉS AMÉLIORÉS POUR LA PRODUCTION D'ISOBUTÈNE À PARTIR D'ACIDE 3-MÉTHYLCROTONIQUE
Abstract
(EN) Described is a method for the production of isobutene from a carbon source characterized in that it comprises: (a) culturing a microorganism capable of producing 3-methylcrotonic acid from a carbon source in a liquid culture medium, thereby producing said 3-methylcrotonic acid so that it accumulates in the liquid culture medium; and (b) enzymatically converting said 3-methylcrotonic acid contained in the liquid culture medium obtained in step (a) into isobutene by: (i) incubating a microorganism expressing an FMN-dependent decarboxylase associated with an FMN prenyl transferase with said liquid culture medium containing 3-methylcrotonic acid obtained in step (a); and/or (ii) incubating an FMN-dependent decarboxylase associated with an FMN prenyl transferase with said liquid culture medium containing 3-methylcrotonic acid obtained in step (a); thereby producing said isobutene; and (c) recovering the produced isobutene.
(FR) Procédé de production d'isobutène à partir d'une source de carbone, caractérisé en ce qu'il comprend les étapes suivantes : (a) culture d'un micro-organisme capable de produire de l'acide 3-méthylcrotonique à partir d'une source de carbone dans un milieu de culture liquide, produisant ainsi ledit acide 3-méthylcrotonique de sorte qu'il s'accumule dans le milieu de culture liquide ; et (b) conversion enzymatique dudit acide 3-méthylcrotonique contenu dans le milieu de culture liquide obtenu dans l'étape (a) en isobutène par : (i) incubation d'un micro-organisme exprimant une décarboxylase dépendante de FMN associée à une FMN prényl transférase avec ledit milieu de culture liquide contenant l'acide 3-méthylcrotonique obtenu à l'étape (a) ; et/ou (ii) incubation d'une décarboxylase dépendante de FMN associée à une FMN prényl transférase avec ledit milieu de culture liquide contenant l'acide 3-méthylcrotonique obtenu à l'étape (a), produisant ainsi ledit isobutène ; et (c) récupération de l'isobutène produit.
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