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1. WO2022133917 - MODIFIED PHOSPHOENOLPYRUVATE CARBOXYLASE AND APPLICATION THEREOF IN INCREASING YIELD OF AMINO ACIDS OF CORYNEBACTERIUM GLUTAMICUM

Publication Number WO/2022/133917
Publication Date 30.06.2022
International Application No. PCT/CN2020/139053
International Filing Date 24.12.2020
IPC
C12N 1/21 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/77 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77for Corynebacterium; for Brevibacterium
C12N 9/88 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88Lyases (4.)
C12N 15/60 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
60Lyases (4)
C12P 13/04 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta-amino acids
C12R 1/15 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
15Corynebacterium
CPC
C12N 15/74
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 15/77
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77for Corynebacterium; for Brevibacterium
C12N 9/88
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12P 13/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta- amino acids
C12R 2001/15
Applicants
  • 武汉远大弘元股份有限公司 WUHAN GRAND HOYO CO., LTD. [CN]/[CN]
Inventors
  • 皮莉 PI, Li
  • 朱程军 ZHU, Chengjun
  • 孙悦 SUN, Yue
  • 林丽春 LIN, Lichun
  • 邢盼盼 XING, Panpan
  • 苏海霞 SU, Haixia
  • 陈磊 CHEN, Lei
  • 唐鹏 TANG, Peng
  • 王炯 WANG, Jiong
  • 左江 ZUO, Jiang
  • 黄治华 HUANG, Zhihua
  • 李晓波 LI, Xiaobo
  • 刘梦洁 LIU, Mengjie
  • 蔡成平 CAI, Chengping
  • 胡小蓉 HU, Xiaorong
  • 王筱蒙 WANG, Xiaomeng
  • 杨磊 YANG, Lei
  • 刘莎 LIU, Sha
Agents
  • 北京清亦华知识产权代理事务所(普通合伙) TSINGYIHUA INTELLECTUAL PROPERTY LLC
Priority Data
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) MODIFIED PHOSPHOENOLPYRUVATE CARBOXYLASE AND APPLICATION THEREOF IN INCREASING YIELD OF AMINO ACIDS OF CORYNEBACTERIUM GLUTAMICUM
(FR) PHOSPHOÉNOLPYRUVATE CARBOXYLASE MODIFIÉE ET SON APPLICATION DANS L'AUGMENTATION DU RENDEMENT D'ACIDES AMINÉS DE CORYNÉBACTÉRIUM GLUTAMICUM
(ZH) 改造的磷酸烯醇丙酮酸羧化酶及其在提高谷氨酸棒杆菌氨基酸产量中的应用
Abstract
(EN) A modified Phosphoenolpyruvate carboxylase. Compared to an amino acid sequence of a wild-type Phosphoenolpyruvate carboxylase, amino acids at bit 771 and/or bit 931 and/or bit 943 of an amino acid sequence of the modified Phosphoenolpyruvate carboxylase are substituted. By performing specific site mutation on the amino acid sequence of the wild-type Phosphoenolpyruvate carboxylase, the modified Phosphoenolpyruvate carboxylase obtained has a great influence on metabolism of an amino acid carbon flow; and expressing a coding gene of the modified Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum can effectively increase the yield of amino acids to achieve good application prospect.
(FR) L'invention concerne une phosphoénolpyruvate carboxylase modifiée. Par comparaison avec une séquence d'acides aminés d'une phosphoénolpyruvate carboxylase de type sauvage, des acides aminés au niveau du bit 771 et/ou du bit 931 et/ou du bit 943 d'une séquence d'acides aminés de la phosphoénolpyruvate carboxylase modifiée sont substitués. En effectuant une mutation de site spécifique sur la séquence d'acides aminés de la phosphoénolpyruvate carboxylase de type sauvage, la phosphoénolpyruvate carboxylase modifiée obtenue a une grande influence sur le métabolisme d'un flux de carbone d'acides aminés; et l'expression d'un gène codant de la phosphoénolpyruvate carboxylase modifiée dans Corynebacterium glutamicum peut augmenter efficacement le rendement des acides aminés pour obtenir de bonnes perspectives d'application.
(ZH) 一种改造的磷酸烯醇丙酮酸羧化酶,与野生型磷酸烯醇丙酮酸羧化酶的氨基酸序列相比,所述改造的磷酸烯醇丙酮酸羧化酶的氨基酸序列的第771位和/或第931位和/或第943位氨基酸被取代。通过将野生型磷酸烯醇丙酮酸羧化酶的氨基酸序列进行特定位点突变,所得到的改造的磷酸烯醇丙酮酸羧化酶对氨基酸碳流量的代谢有很大影响,利用该改造的磷酸烯醇丙酮酸羧化酶的编码基因在谷氨酸棒杆菌中表达,可以高效提高氨基酸产量,应用前景好。
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