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1. WO2022208327 - SPATIAL MAPPING BY SERIAL PRIMER EXTENSION

Publication Number WO/2022/208327
Publication Date 06.10.2022
International Application No. PCT/IB2022/052862
International Filing Date 29.03.2022
IPC
C12Q 1/6806 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
C12Q 1/6837 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
6837using probe arrays or probe chips
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6837
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
6837using probe arrays or probe chips
Applicants
  • PIXELGEN TECHNOLOGIES AB [SE]/[SE]
Inventors
  • FREDRIKSSON, Simon
  • KARLSSON, Filip
Priority Data
63/168,13230.03.2021US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SPATIAL MAPPING BY SERIAL PRIMER EXTENSION
(FR) CARTOGRAPHIE SPATIALE PAR EXTENSION D'AMORCE EN SÉRIE
Abstract
(EN) Provided herein is probe system comprising: a population of nucleic acid molecules that have an extendible end, a first set of barcoded particles that each have a nucleotide sequence comprising: (i) a binding sequence that is complementary to the extendible end of the nucleic acid molecules, (ii) a unique particle identifier sequence, and (iii) a first template sequence, and a second set of barcoded particles that each have a nucleotide sequence comprising: (i) the first template sequence and (ii) a unique particle identifier sequence. In use, extension of the nucleic acid molecules using the first set of barcoded particles of as a template produces extensions products that contain the complement of a unique particle identifier sequence of a particle and the complement of the first template sequence. Methods of using the probe system to map binding events in or on a cellular sample are also provided.
(FR) La présente invention concerne un système de sonde comprenant les éléments suivants : une population de molécules d'acide nucléique possédant une extrémité extensible, un premier ensemble de particules à code-barres possédant chacune une séquence nucléotidique comprenant : (i) une séquence de liaison complémentaire à l'extrémité extensible des molécules d'acide nucléique, (ii) une séquence identificatrice de particule unique, et (iii) une première séquence matrice, et un second ensemble de particules à code-barres possédant chacune une séquence nucléotidique comprenant : (i) la première séquence matrice et (ii) une séquence identificatrice de particule unique. Lors de l'utilisation, l'extension des molécules d'acide nucléique en utilisant le premier ensemble de particules à code-barres comme matrice produit des produits d'extension contenant le complément d'une séquence d'identification de particule unique d'une particule et le complément de la première séquence matrice. La présente invention concerne également des procédés d'utilisation du système de sondes pour cartographier les événements de liaison dans ou sur un échantillon cellulaire.
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