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IC:C12P19/34

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Analysis

1 / 2,202
1.WO/2020/185811FED-BATCH IN VITRO TRANSCRIPTION PROCESS
WO - 17.09.2020
Int.Class C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
 Appl.No PCT/US2020/021955 Applicant MODERNATX, INC. Inventor ELICH, Joseph
The present disclosure provides methods of in vitro transcribing a ribonucleic acid (RNA) of interest. In some embodiments, such methods include determining consumption rates of nucleoside triphosphates (NTPs).
2.WO/2020/185831EXPRESSION VECTOR
WO - 17.09.2020
Int.Class C12N 15/76
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
76for Actinomyces; for Streptomyces
 Appl.No PCT/US2020/021986 Applicant VARIGEN BIOSCIENCES CORPORATION Inventor STANKEY, Robert Joseph
Disclosed herein are recombinant methods of activating expression of one or more biosynthetic gene clusters comprising more than one gene, the method comprising a recombinant DNA expression vector that possess two opposable inducible promoters that drives expression of a biosynthetic gene cluster exogenously from outside of the cluster to produce polyketides or non-ribosomal peptides in a heterologous host.
3.WO/2020/181072MESOPHILIC ARGONAUTE SYSTEMS AND USES THEREOF
WO - 10.09.2020
Int.Class C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
 Appl.No PCT/US2020/021163 Applicant THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY Inventor QI, Lei S.
Constructs comprising Argonautes and neighboring genes are disclosed for use in gene editing. Disclosed are also compositions and methods utilizing these Argonautes and neighboring genes. Also disclosed are the methods of making and using the Argonautes and neighboring genes in treating various diseases, conditions, and cancer.
4.WO/2020/181129HIGH ACCURACY NANOPORE-BASED SINGLE MOLECULE SEQUENCING BY SYNTHESIS WITH TAGGED NUCLEOTIDES
WO - 10.09.2020
Int.Class G01N 33/487
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
 Appl.No PCT/US2020/021253 Applicant THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK Inventor JU, Jingyue
Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.
5.WO/2020/176362COMPOSITIONS AND METHODS FOR NEXT GENERATION SEQUENCING
WO - 03.09.2020
Int.Class C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
 Appl.No PCT/US2020/019371 Applicant TWIST BIOSCIENCE CORPORATION Inventor GANTT, Richard
Provided herein are compositions and methods for next generation sequencing using universal polynucleotide adapters. Further provided are universal adapters using locked nucleic acids or bridged nucleic acids. Further provided are barcoded primers of reduced length for extension of universal adapters. Further provided herein are universal adapter blockers.
6.WO/2020/172199GUIDE STRAND LIBRARY CONSTRUCTION AND METHODS OF USE THEREOF
WO - 27.08.2020
Int.Class C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
 Appl.No PCT/US2020/018701 Applicant PIONEER BIOLABS, LLC Inventor YATES, Joshua
An improved method for the rapid and efficient production of guide strand libraries is disclosed. Also included are kits comprising reagents suitable for practicing the method. Embodiments may include a polynucleotide comprising a sequence encoding for a constant region comprising a protein binding segment of an RNA component of a CRISPR complex. The sequence may comprise a non-palindromic recognition sequence for a first restriction enzyme having methyltransferase activity and configured to cleave at least 17 nucleotides outside of the non-palindromic recognition sequence, wherein at least one base of the non-palindromic recognition sequence is configured to be methylated by the first restriction enzyme, thereby blocking endonuclease activity of the first restriction enzyme.
7.WO/2020/171092METHOD FOR PRODUCING MODIFIED OLIGONUCLEOTIDE INCLUDING COMPLEMENTARY SEQUENCE
WO - 27.08.2020
Int.Class C07H 21/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02with ribosyl as saccharide radical
 Appl.No PCT/JP2020/006366 Applicant AJINOMOTO CO., INC. Inventor TAKAHASHI, Daisuke
The present invention provides an efficient method for producing an oligonucleotide including a complementary portion, such as siRNA and heteroduplex oligonucleotide. More specifically, this method is for producing a modified oligonucleotide including a 11 to 27 base-long complementary portion, the method including treating a total of at least four oligonucleotide material fragments in the presence of an oligonucleotide ligase to generate the modified oligonucleotide. In this method, the total of at least four oligonucleotide material fragments each correspond to an oligonucleotide material fragment that can be obtained by cleaving this modified oligonucleotide at a fragment linking region, where conditions (i) to (v) are satisfied. Condition (i): at least one fragment linking region is present in each strand of the complementary portion, and a total of at least two fragment linking regions are present in the modified oligonucleotide. Condition (ii): when this modified oligonucleotide is cleaved at a fragment linking region, a protruding end is formed in the complementary portion, and the protruding end is 1 to 10 base long. Condition (iii): at least one oligonucleotide material fragment includes a modified nucleotide. Condition (iv): of the total of at least four oligonucleotide material fragments, four oligonucleotide material fragments include a 5 to 25 base-long complementary portion. Condition (v): the total of the base length corresponding to each of the strands of the complementary portion of a oligonucleotide material fragment is 11 to 27 bases.
8.WO/2020/171949MULTIPLEX NUCLEIC ACID AMPLIFICATION AND LIBRARY PREPARATION
WO - 27.08.2020
Int.Class C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
 Appl.No PCT/US2020/016655 Applicant ROMA, Gianluca Inventor ROMA, Gianluca
The present teachings relate to the use of multiplex PCR amplification to enrich multiple targets of interest while reducing post-amplification by-products via controlled primer digestion for use during the preparation of massively parallel next-generation DNA sequencing library construction. In one aspect, the present teachings relate to specific primer design, and in another aspect, the present teachings relate to an overall optimization of workflow that increases the efficiency of next-generation sequencing by targeting only user-defined regions of interest and substantially reducing or eliminating contaminating genomic DNA, and other interfering by-products of targeted amplification or unused reactants. Particularly, processes and kits are disclosed for preparing a plurality of multiplex amplification products for targeted next generation-sequencing providing reduced background noise.
9.2780827Partículas de transducción no replicativas y sistemas indicadores basados en partículas de transducción
ES - 27.08.2020
Int.Class G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
 Appl.No 14775849 Applicant Geneweave Biosciences Inc. Inventor REY, Diego, Ariel
10.20200270664STABILIZED REDUCING AGENTS AND METHODS USING SAME
US - 27.08.2020
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
 Appl.No 16809280 Applicant 10X Genomics, Inc. Inventor Lawrence Greenfield

The disclosure provides stabilized reducing agents and methods for using them in sample preparation. Stabilized reducing agents described herein provide easy-to-use replacement reducing agents for reducing agents that undergo side-reactions that can render them ineffective as reducing agents and/or decrease the concentration of available reducing agent. In some cases, a stabilized reducing agent is an activatable reducing agent that can be activated upon application of a stimulus to the reducing agent.

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