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1.WO/2020/235574THREE-LAYER LAMINATE CONSTITUTED OF EXTRACELLULAR MATRIX, SHEET-SHAPED CELL CULTURE, AND BIODEGRADABLE GEL IN THAT ORDER
WO 26.11.2020
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/JP2020/019847 Applicant TERUMO KABUSHIKI KAISHA Inventor OHASHI, Fumiya
The present invention addresses the problem of providing a laminate that is easier to manipulate, in which when a thrombin solution is sprayed, damage to a sheet-shaped cell culture due to the spraying pressure is reduced, and a fibrin gel is formed on one surface of the sheet-shaped cell culture. This problem is solved by: in the production of a sheet-shaped cell culture in which cells are cultured on a temperature-responsive cell culture substrate, fibrinogen is instilled on a sheet-shaped cell culture having an extracellular matrix on the bottom surface, and furthermore, a thrombin solution is sprayed onto the sheet-shaped cell culture from an oblique direction; and by providing a laminate having a fibrin gel layer only on one surface of the sheet-shaped cell culture, and having an extracellular matrix layer on the other surface.
2.WO/2020/230886RESIN FILM FORMED OF SCAFFOLD MATERIAL FOR CELL CULTURE, CARRIER FOR CELL CULTURE AND CONTAINER FOR CELL CULTURE
WO 19.11.2020
Int.Class C07K 5/11
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides having up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
10Tetrapeptides
11the side chain of the first amino acid containing more amino groups than carboxyl groups, or derivatives thereof, e.g. Lys, Arg
Appl.No PCT/JP2020/019416 Applicant SEKISUI CHEMICAL CO., LTD. Inventor IGUCHI, Hiroki
Provided is a resin film formed of a scaffold material for cell culture, said resin film enabling excellent fixation of cells after seeding and thus contributing to an increase in the cell proliferation rate. The resin film is formed of a scaffold material for cell culture, wherein: the scaffold material for cell culture comprises a synthetic resin; the resin film has a phase-separation structure containing at least a first phase and a second phase; and the ratio of the surface area of one of the first and second phases to the total surface area is 0.01-0.95 inclusive.
3.WO/2020/230885CELL CULTURING SCAFFOLDING MATERIAL AND CELL CULTURING VESSEL
WO 19.11.2020
Int.Class C07K 5/11
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides having up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
10Tetrapeptides
11the side chain of the first amino acid containing more amino groups than carboxyl groups, or derivatives thereof, e.g. Lys, Arg
Appl.No PCT/JP2020/019415 Applicant SEKISUI CHEMICAL CO., LTD. Inventor TAKAKURA, Kenta
Provided is a cell culturing scaffolding material having excellent extensibility of a pseudopodium and an excellent cell-proliferation property. A cell culturing scaffolding material according to the present invention includes a peptide-containing polyvinyl alcohol derivative having a polyvinyl alcohol derivative part and a peptide part, and has a hydroxyl value of at most 1100 mgKOH/g.
4.WO/2020/227934BIOLOGICAL PRODUCT MANUFACTURING METHOD, BIOLOGICAL PRODUCT, AND APPLICATION
WO 19.11.2020
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/CN2019/086884 Applicant SHENZHEN INSTITUTE OF ADVANCED TECHNOLOGY,SHENZHEN (CN) Inventor WANG, Pengyuan
The present application relates to the technical field of biological materials, and provides a biological product manufacturing method, a biological product, and an application. In the biological product manufacturing method provided by the present application, a biological product is obtained by pouring a micro-particle solution, an organic nano-particle solution, and optional water into a same culture well of a culture plate to form a micro-nano particle mixture, then drying the micro-nano particle mixture in the culture plate, and finally fixing a multi-level micro-nano structure arranged in the culture well. According to the manufacturing method, a large number of multi-level micro-nano structures having different chemical compositions and surface topologies are quickly prepared in a culture plate by means of particle self-assembly technology, and the manufactured biological product achieves the combination of the culture plate and the multi-level micro-nano structure, thereby providing a basis for the application thereof in biomedicine. The present application further provides a biological product manufactured by the biological product manufacturing method, and an application of the biological product in the field of cell culture such as cell adhesion, proliferation, and differentiation.
5.WO/2020/230884CELL CULTURING SCAFFOLD MATERIAL AND CELL CULTURING CONTAINER
WO 19.11.2020
Int.Class C07K 5/11
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
5Peptides having up to four amino acids in a fully defined sequence; Derivatives thereof
04containing only normal peptide links
10Tetrapeptides
11the side chain of the first amino acid containing more amino groups than carboxyl groups, or derivatives thereof, e.g. Lys, Arg
Appl.No PCT/JP2020/019414 Applicant SEKISUI CHEMICAL CO., LTD. Inventor KOBAYASHI, Daigo
Provided is a cell culturing scaffold material having excellent adhesiveness for cells. This cell culturing scaffold material contains a peptide-including polyvinyl alcohol derivative that has a polyvinyl alcohol derivative part and a peptide part, wherein the peptide part has a cyclic peptide backbone.
6.WO/2020/231833A GLOMERULUS ON A CHIP TO RECAPITULATE GLOMERULAR FILTRATION BARRIER
WO 19.11.2020
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/US2020/032163 Applicant CHILDREN'S HOSPITAL LOS ANGELES Inventor PERIN, Laura
A glomerulus on a chip (GOAC) to recapitulate the human glomerular filtration barrier, the structure responsible for filtering the blood and preventing the loss of proteins, is provided using human podocytes and glomerular endothelial cells seeded into microfluidic chips. In long-term cultures, cells maintain their morphology, form capillary-like structures and express slit diaphragm proteins. This system recapitulates functions and structure of the glomerulus, including permselectivity. When exposed to sera from patients with anti-podocyte autoantibodies, the chips show albuminuria proportional to patients' proteinuria, phenomenon not observed with sera from healthy controls or individuals with primary podocyte defects. Also shown is its applicability for renal disease modeling and drug testing.
7.WO/2020/227772MICROFLUIDIC DEVICE AND METHOD OF USE FOR CELL CULTURE
WO 19.11.2020
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/AU2020/050473 Applicant NEWSOUTH INNOVATIONS PTY LIMITED Inventor NORDON, Robert, Ernest
The invention relates to a multi-layer microfluidic bioreactor and methods for culturing cells. A grooved semipermeable substrate can capture and grow cells using perfusion culture methods. Microfluidic geometry directs flow over grooves for cell expansion 5 and along grooves for cell harvesting. Some embodiments are optimised for high density and automated processing for growth of cells.
8.WO/2020/229650INCUBATION SYSTEM
WO 19.11.2020
Int.Class C12M 1/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
26Inoculator or sampler
32multiple field or continuous type
Appl.No PCT/EP2020/063581 Applicant EVORION BIOTECHNOLOGIES GMBH Inventor KLEINE-BRÜGGENEY, Hans
The invention relates to an incubation system (26) comprising a preferably multi-layer substrate (1) and a container (27). The substrate (1) is received in the container (27) such that the container (27) and the substrate (1) are in fluid communication via at least one interface. The substrate (1) is fastened to the container (27) such that the at least one interface for the exchange of fluid between the container (27) and the substrate (1) is leakproof.
9.20200362310METHOD FOR PRODUCING SHEET-SHAPED CELL CULTURE
US 19.11.2020
Int.Class C12N 5/077
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
Appl.No 16985630 Applicant TERUMO KABUSHIKI KAISHA Inventor Tadashi SAMESHIMA

Disclosed are methods of preparing a high-quality sheet-shaped cell culture by preventing breakage of a sheet-shaped cell culture that is caused by adhesion, sticking and/or shrinkage of the sheet-shaped cell culture as a result of detaching, transferring or preserving the sheet-shaped cell culture. Also disclosed are methods of detaching, cleaning, and/or preserving a sheet-shaped cell culture, including soaking a sheet-shaped cell culture in a low nutrient isotonic solution.

10.20200363300PULSE-MODULATED PERIODIC BACKFLUSH FOR CLEARANCE OF FOULING LAYERS IN DEAD-END FILTRATION SYSTEMS
US 19.11.2020
Int.Class G01N 1/34
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
34Purifying; Cleaning
Appl.No 16965111 Applicant Georgia Tech Research Corporation Inventor Aaron Enten

Embodiments of the present disclosure relate generally to dead-end filtration systems and, more particularly, to pulse-modulated periodic backflush systems and methods for clearing fouling layers in dead-end filtration systems. In some embodiments, a controller may control the flow of fluid in the system from cycling from a forward flow to a reverse flow. In some embodiments, the controller may cycle from forward to reverse flow based on a volumetric flow ratio. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing the break of cake in a dead-end filtration system. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing recovery percentages of targeted particles.