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1. WO2020233958 - PURIFICATION OF OLIGOSACCHARIDES FROM A FERMENTATION BROTH BY USING FILTRATION

Publication Number WO/2020/233958
Publication Date 26.11.2020
International Application No. PCT/EP2020/061888
International Filing Date 29.04.2020
IPC
C07H 1/08 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1Processes for the preparation of sugar derivatives
06Separation; Purification
08from natural products
C07H 3/06 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
3Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
06Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
A61K 31/702 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
702Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
C12P 19/14 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
14produced by the action of a carbohydrase, e.g. by alpha-amylase
A23L 5/00 2016.01
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D91; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
5Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
A23L 29/30 2016.01
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D91; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
29Foods or foodstuffs containing additives; Preparation or treatment thereof
30containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
CPC
A23L 29/269
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT
29Foods or foodstuffs containing additives
20containing gelling or thickening agents
269of microbial origin, e.g. xanthan or dextran
A23L 5/00
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT
5Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
C07H 1/08
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
1Processes for the preparation of sugar derivatives
06Separation; Purification
08from natural products
C07H 3/06
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
3Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
06Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
C12P 19/14
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
14produced by the action of a carbohydrase ; (EC 3.2.x); , e.g. by alpha-amylase ; , e.g. by cellulase, hemicellulase
Applicants
  • JENNEWEIN BIOTECHNOLOGIE GMBH [DE]/[DE]
Inventors
  • JENNEWEIN, Stefan
  • KRAHN, Jan Henrik
  • HELFRICH, Markus
Priority Data
19175716.021.05.2019EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PURIFICATION OF OLIGOSACCHARIDES FROM A FERMENTATION BROTH BY USING FILTRATION
(FR) PURIFICATION D'OLIGOSACCHARIDES À PARTIR D'UN BOUILLON DE FERMENTATION PAR FILTRATION
Abstract
(EN)
A method for the purification of an oligosaccharide, preferably a neutral human milk oligosaccharide (HMO) or a sialylated HMO, from a fermentation broth, the method comprising: - providing a fermentation broth which contains the oligosaccharide of interest, biomass, microbial cells and carbohydrates other than the oligosaccharide of interest; - removing the microbial cells from the fermentation broth, thereby providing a process stream; - subjecting the process stream to a first filtration step using a nanofiltration membrane, thereby providing a filtrate which contains the oligosaccharide of interest; - subjecting the filtrate to a second filtration step using a nanofiltration membrane, thereby providing a retentate which contains the oligosaccharide of interest; and - removing salts from the process stream using electrodialysis thereby providing a purified preparation of the oligosaccharide of interest.
(FR)
L'invention concerne un procédé de purification d'un oligosaccharide, de préférence un oligosaccharide de lait humain neutre (HMO) ou un HMO sialylé, à partir d'un bouillon de fermentation, le procédé comprenant les étapes consistant à : - fournir un bouillon de fermentation qui contient l'oligosaccharide d'intérêt, de la biomasse, des cellules microbiennes et des glucides autres que l'oligosaccharide d'intérêt ; - éliminer les cellules microbiennes du bouillon de fermentation, fournissant ainsi un flux de traitement ; - soumettre le flux de traitement à une première étape de filtration à l'aide d'une membrane de nanofiltration, ce qui permet d'obtenir un filtrat qui contient l'oligosaccharide d'intérêt ; - soumettre le filtrat à une seconde étape de filtration à l'aide d'une membrane de nanofiltration, ce qui permet d'obtenir un rétentat qui contient l'oligosaccharide d'intérêt ; et - éliminer les sels du flux de traitement à l'aide d'une électrodialyse, ce qui permet d'obtenir une préparation purifiée de l'oligosaccharide d'intérêt.
Also published as
Latest bibliographic data on file with the International Bureau