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1. WO2020185627 - HUMAN GUT MICROBIOME-DERIVED BIOSYNTHETIC ENZYMES FOR PRODUCTION OF FATTY ACID AMIDES

Publication Number WO/2020/185627
Publication Date 17.09.2020
International Application No. PCT/US2020/021578
International Filing Date 06.03.2020
IPC
C12P 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
1Preparation of compounds or compositions, not provided for in groups C12P3/-C12P39/121; General processes for the preparation of compounds or compositions by using microorganisms or enzymes
04by using bacteria
C12P 7/64 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C07K 14/33 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
33from Clostridium (G)
CPC
C07K 14/33
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
33from Clostridium (G)
C12P 13/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
02Amides, e.g. chloramphenicol ; or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
C12Y 203/01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
203Acyltransferases (2.3)
01transferring groups other than amino-acyl groups (2.3.1)
C12Y 203/01041
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
203Acyltransferases (2.3)
01transferring groups other than amino-acyl groups (2.3.1)
01041Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
C12Y 204/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
204Glycosyltransferases (2.4)
C12Y 207/07006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
207Transferases transferring phosphorus-containing groups (2.7)
07Nucleotidyltransferases (2.7.7)
07006DNA-directed RNA polymerase (2.7.7.6)
Applicants
  • MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US]/[US]
Inventors
  • VOIGT, Christopher, A.
  • CHANG, Fang-Yuan
Agents
  • LOCKHART, Helen, C.
  • ATTISHA, Michael, J.
  • AMUNDSEN, Eric, L.
  • BAKER, C., Hunter
  • ALAM, Saad
Priority Data
62/816,02108.03.2019US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) HUMAN GUT MICROBIOME-DERIVED BIOSYNTHETIC ENZYMES FOR PRODUCTION OF FATTY ACID AMIDES
(FR) ENZYMES BIOSYNTHÉTIQUES DÉRIVÉES DU MICROBIOME INTESTINAL HUMAIN POUR LA PRODUCTION D'AMIDES D'ACIDES GRAS
Abstract
(EN)
Disclosed herein, in some embodiments, are vectors encoding biosynthetic enzymes from gut microbiome-derived bacterium (e.g., Clostridia enzymes), engineered cells comprising the vectors, and methods of using biosynthetic enzymes from gut microbiome- derived bacterium (e.g., Clostridia enzymes) to produce fatty acid amides.
(FR)
Dans certains modes de réalisation, l'invention concerne des vecteurs codant des enzymes biosynthétiques issues d'une bactérie dérivée du microbiome intestinal (p. ex., des enzymes Clostridia), des cellules modifiées comprenant les vecteurs, et des procédés d'utilisation d'enzymes biosynthétiques issues d'une bactérie dérivée du microbiome intestinal (p. ex., les enzymes Clostridia) pour produire des amides d'acides gras.
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