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1. WO2020099225 - CULTURE MEDIUM COMPRISING KETO ACIDS

Publication Number WO/2020/099225
Publication Date 22.05.2020
International Application No. PCT/EP2019/080466
International Filing Date 07.11.2019
IPC
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C12P 13/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
Applicants
  • EVONIK OPERATIONS GMBH [DE]/[DE]
Inventors
  • KESSLER, Christian
  • KARAU, Andreas
Agents
  • EVONIK PATENT ASSOCIATION
Priority Data
18205586.312.11.2018EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CULTURE MEDIUM COMPRISING KETO ACIDS
(FR) MILIEU DE CULTURE COMPRENANT DES CÉTO-ACIDES
Abstract
(EN)
The invention relates to a culture medium, preferably a cell culture medium, comprising at least one keto acid selected from keto leucine, keto valine, keto isoleucine and keto phenylalanine and/or a salt of these keto acids. The invention further relates to the use of a culture medium of the invention for culturing cells, preferably plant cells, animal cells or mammalian cells. Another aspect of the invention relates to a method of manufacturing a cell culture product comprising the steps of (i) providing a cell capable of producing said cell culture product; (ii) contacting said cell with a culture medium according to the invention; and (iii) obtaining said cell culture product from said culture medium or from said cell.
(FR)
L'invention concerne un milieu de culture, de préférence un milieu de culture cellulaire, comprenant au moins un céto-acide choisi parmi la céto-leucine, céto-valine, céto-isoleucine et céto-phénylalanine et/ou un sel de ces céto-acides. L'invention concerne en outre l'utilisation d'un milieu de culture de l'invention pour la culture de cellules, de préférence de cellules végétales, de cellules animales ou de cellules de mammifère. Un autre aspect de l'invention concerne un procédé de fabrication d'un produit de culture cellulaire comprenant les étapes de (i) fourniture d'une cellule apte à produire ledit produit de culture cellulaire ; (ii) mise en contact de ladite cellule avec un milieu de culture selon l'invention ; et (iii) obtention dudit produit de culture cellulaire à partir dudit milieu de culture ou de ladite cellule.
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