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1. WO2020061524 - METHODS OF MULTIPLEX dPCR ASSAYS AND SHORT-READ SEQUENCING ASSAYS

Publication Number WO/2020/061524
Publication Date 26.03.2020
International Application No. PCT/US2019/052268
International Filing Date 20.09.2019
IPC
C12Q 1/6816 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
C12Q 1/6818 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
6818involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6827 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6827for detection of mutation or polymorphism
C40B 30/04 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
30Methods of screening libraries
04by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
Applicants
  • 13.8, INC. [US/US]; 2275 East Bayshore Road, Suite 108 Palo Alto, California 94303, US
Inventors
  • FAN, Christina; US
Agents
  • HOLZAPFEL, Keli L.; US
Priority Data
62/734,03520.09.2018US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF MULTIPLEX dPCR ASSAYS AND SHORT-READ SEQUENCING ASSAYS
(FR) PROCÉDÉS DE DOSAGES DPCR MULTIPLEX ET DOSAGES DE SÉQUENÇAGE À LECTURE COURTE
Abstract
(EN)
Provided herein are methods of multiplex digital PCR assays and short-read sequencing assays. The methods described herein utilize high-throughput sample distribution techniques, fluorescent channel monitoring, and nucleic acid sequence melting curve analysis.
(FR)
La présente invention concerne des procédés de dosages PCR numériques multiplex et des dosages de séquençage à lecture courte. Les procédés décrits ici utilisent des techniques de distribution d'échantillon à haut débit, une surveillance de canal fluorescent et une analyse de courbe de fusion de séquence d'acide nucléique.
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